       Document 0955
 DOCN  M9650955
 TI    Activation of the human immunodeficiency virus long terminal repeat by
       abrasion of the skin in transgenic mice.
 DT    9505
 AU    Morrey JD; Bourn SM; Morris JL; Bunch TD; Sidwell RW; Department of
       Animal, Dairy and Veterinary Sciences, Utah State; University, Logan
       84322-4700, USA.
 SO    Intervirology. 1994;37(6):315-20. Unique Identifier : AIDSLINE
       MED/96022718
 AB    Mechanical wounding was shown to activate the human immunodeficiency
       virus type 1 (HIV-1) long terminal repeat (LTR) in the skin of
       transgenic mice. Both noninvasive rubbing and scratching of the skin
       resulted in a range of 4- to 44-fold increased levels of luciferase
       reporter gene activities when assayed 24-48 h after wounding. Moreover,
       long-term noninvasive rubbing each day for 17 days resulted in similar
       increased levels of luciferase activity. Experiments were done to
       determine whether the HIV-1 LTR-luciferase transgene might be activated
       when pups were nursed on the mammary tissues of transgenic mice.
       Luciferase reporter gene activity in mammary glands skin following
       nursing was significantly higher than in skin from non-pregnant
       transgenic mice or transgenic mice 20 days post-conception, which
       suggests that the natural abrasive action of nursing resulted in
       activation of the LTR. These results may have implications for sexual
       transmission and maternal-to-infant transmission of HIV-1.
 DE    Animal  Female  Gene Expression Regulation, Viral  Genes, Reporter
       Human  HIV Long Terminal Repeat/*GENETICS  HIV-1/*GROWTH & DEVELOPMENT
       Luciferase/BIOSYNTHESIS  Mammae  Mice  Mice, Transgenic  Pregnancy
       *Skin/INJURIES  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Transgenes  Virus Activation/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

