       Document 0952
 DOCN  M9650952
 TI    Comparison of two alternative microdilution procedures with the National
       Committee for Clinical Laboratory Standards reference macrodilution
       method M27-P for in vitro testing of fluconazole-resistant and
       -susceptible isolates of Candida albicans.
 DT    9505
 AU    Espinel-Ingroff A; Rodriguez-Tudela JL; Martinez-Suarez JV; Medical
       College of Virginia, Virginia Commonwealth University,; Richmond
       23298-0049, USA.
 SO    J Clin Microbiol. 1995 Dec;33(12):3154-8. Unique Identifier : AIDSLINE
       MED/96156118
 AB    The National Committee for Clinical Laboratory Standards has proposed a
       reference broth macrodilution method for in vitro antifungal
       susceptibility testing of yeasts (the M27-P method). This method is
       cumbersome and time-consuming and includes MIC endpoint determination by
       the visual and subjective inspection of growth inhibition after 48 h of
       incubation. Two alternative microdilution procedures for MIC endpoint
       determination, a spectrophotometric MIC endpoint test that evaluates 80%
       growth inhibition by the drug and a colorimetric method with an
       oxidation-reduction indicator (Alamar Blue), were compared with the
       M27-P method for fluconazole susceptibility testing of 45 susceptible
       and resistant isolates of Candida albicans. The spectrophotometric
       method was performed with RPMI 1640 medium with 2% glucose, and the
       other two tests were performed with plain RPMI 1640 medium. All tests
       were incubated at 35 degrees C. Excellent agreement was demonstrated
       between the M27-P method and both 24-h microdilution tests (97.7%) as
       well as between the two microdilution tests (95.5%). Also, there was
       agreement in the detection in vivo of fluconazole resistance by the
       three methods. These preliminary data indicate that both microdilution
       methods may serve as less subjective alternatives to the M27-P method
       for the determination of fluconazole MIC endpoints.
 DE    Antifungal Agents/*PHARMACOLOGY  AIDS-Related Opportunistic
       Infections/COMPLICATIONS/DRUG THERAPY/  MICROBIOLOGY  Candida
       albicans/*DRUG EFFECTS/ISOLATION & PURIF  Candidiasis,
       Oral/COMPLICATIONS/DRUG THERAPY/MICROBIOLOGY  Colorimetry  Comparative
       Study  Drug Resistance, Microbial  Evaluation Studies
       Fluconazole/*PHARMACOLOGY  Human  Microbial Sensitivity
       Tests/*METHODS/STANDARDS  Spectrophotometry  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

