       Document 0947
 DOCN  M9650947
 TI    Simple enzyme immunoassay for titration of antibodies to the CD4-binding
       site of human immunodeficiency virus type 1 gp120.
 DT    9505
 AU    Turbica I; Posner M; Bruck C; Barin F; Departement de Microbiologie
       Medicale et Moleculaire, Unite; de Recherche Associee Centre National de
       la Recherche; Scientifique 1334, Centre Hospitalier Universitaire
       Bretonneau,; Tours, France.
 SO    J Clin Microbiol. 1995 Dec;33(12):3319-23. Unique Identifier : AIDSLINE
       MED/96156153
 AB    We report the development of an immunoassay for the titration of
       antibody to the CD4-binding site (CD4BS) of the human immunodeficiency
       virus type 1 (HIV-1) surface glycoprotein gp120. This assay is a
       competitive enzyme-linked immunosorbent assay in which serum antibodies
       compete with labeled F105, a human monoclonal antibody whose
       corresponding epitope overlaps the conformation-dependent CD4BS, for
       binding to purified recombinant gp120 coated on a solid phase.
       Ninety-nine percent (109 of 110) of HIV-1-positive French patients and
       91% (51 of 56) of HIV-1-positive African patients had CD4BS antibodies,
       indicating that the conformational CD4BS epitope is well conserved among
       different subtypes of HIV-1. Titers of CD4BS antibodies according to
       clinical status appeared to be not statistically different. A
       longitudinal study in 21 seroconverters showed that, for the majority of
       individuals, CD4BS antibodies appeared early and persisted at relatively
       high titers for several years. None of 21 HIV-2-seropositive patients
       had CD4BS antibodies in our assay, suggesting that the antibodies
       produced during HIV-2 infection are not cross-reactive with the CD4BS of
       HIV-1 gp120.
 DE    Amino Acid Sequence  Antigens, CD4/*METABOLISM  Binding Sites/GENETICS
       Binding, Competitive  Consensus Sequence  Cross Reactions  Enzyme-Linked
       Immunosorbent Assay/*METHODS  Human  HIV Antibodies/*ANALYSIS  HIV
       Envelope Protein gp120/GENETICS/*IMMUNOLOGY/METABOLISM  HIV
       Infections/IMMUNOLOGY  HIV-1/GENETICS/*IMMUNOLOGY  HIV-2/IMMUNOLOGY
       Molecular Sequence Data  Sequence Homology, Amino Acid  Support,
       Non-U.S. Gov't  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

