       Document 0946
 DOCN  M9650946
 TI    Epstein-Barr virus (EBV) DNA in saliva and EBV serology of
       HIV-1-infected persons with and without hairy leukoplakia.
 DT    9505
 AU    Lucht E; Biberfeld P; Linde A; Department of Clinical Virology, Swedish
       Institute for Infectious; Disease Control, Stockholm, Sweden.
 SO    J Infect. 1995 Nov;31(3):189-94. Unique Identifier : AIDSLINE
       MED/96163962
 AB    Secretion of Epstein-Barr virus (EBV) in saliva, as well as serum
       antibody titres against various EBV antigens, were analyzed in respect
       of (1) 15 HIV-1-infected patients with oral hairy leukoplakia proven to
       contain EBV by in situ hybridization, (2) 45 HIV-1 infected patients
       without hairy leukoplakia, (3) 10 HIV-1 infected patients treated with
       acyclovir or foscarnet and (4) 21 healthy controls. The numbers of CD4+
       cells in the peripheral blood were also recorded. The HIV-1 infected
       patients were at various stages of HIV-1-associated disease. Excretion
       of EBV DNA in the saliva was determined by means of the polymerase chain
       reaction (PCR) while the amount of EBV DNA in positive samples was
       estimated by repeated titrations. The frequency of shedding of EBV DNA
       increased from 33% in healthy controls to 78% in asymptomatic HIV-1
       infected persons, but did not increase significantly with progression of
       HIV-1-associated disease. The titres of EBV DNA in saliva correlated
       inversely and significantly with the number of CD4+ cells in the
       peripheral blood. All patients with hairy leukoplakia shed by EBV DNA in
       their saliva but the titres were not significantly higher than those of
       other HIV-1 infected persons. The serum titres of antibodies against EBV
       nuclear antigen 1 (EBNA-1) correlated positively and significantly with
       the CD4+ cell count in the peripheral blood. EBNA-1 IgG antibody in the
       serum was also significantly lower in symptomatic than in asymptomatic
       HIV-1 infected persons. There were, however, no significant differences
       in serum antibodies to various EBV antigens between patients with and
       without hairy leukoplakia.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS/IMMUNOLOGY
       Antibodies, Viral/ANALYSIS/IMMUNOLOGY  Antigens,
       Viral/ANALYSIS/IMMUNOLOGY  Comparative Study  CD4 Lymphocyte Count  DNA,
       Viral/*ANALYSIS  Enzyme-Linked Immunosorbent Assay  Female  Herpesvirus
       4, Human/IMMUNOLOGY/*ISOLATION & PURIF  Human  HIV-1/*ISOLATION & PURIF
       Leukoplakia, Hairy/*COMPLICATIONS/DIAGNOSIS  Male  Polymerase Chain
       Reaction  Saliva/*VIROLOGY  Support, Non-U.S. Gov't  CLINICAL TRIAL
       JOURNAL ARTICLE  RANDOMIZED CONTROLLED TRIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

