       Document 0930
 DOCN  M9650930
 TI    Detection and characterization by immunoblot analysis of potentially
       diagnostic Leishmania infantum polypeptides in human visceral
       leishmaniasis.
 DT    9505
 AU    Cardenosa N; Riera C; Cortes P; March F; Munoz C; Portus M; Prats G;
       Departament de Microbiologia, Hospital de la Santa Creu i Sant; Pau,
       Universitat Autonoma de Barcelona, Spain.
 SO    Parasite Immunol. 1995 Oct;17(10):509-16. Unique Identifier : AIDSLINE
       MED/96164305
 AB    Humoral immune responses were studied in 53 sera from 18 patients with
       visceral leishmaniasis by immunoblot analysis. Sera from visceral
       leishmaniasis patients recognized antigens with molecular weights
       ranging from < 14 kDa to more than 100 kDa. Bands ranging between 49 and
       < 14 kDa were the most specific. The 40, 33 and 17 kDa antigens were
       recognized by 90%, 79% and 79% of the patients sera, respectively. Sera
       from one patient with Chagas' disease identified 8 of 11 antigens of the
       specific region. Treatment with periodate eliminated the cross-reaction
       in three of these antigens (40, 29, 26 kDa). The study of serial sera
       collected from the different patients showed a decrease in intensity or
       dissappearance in some of the diagnostic bands, particularly the 17 kDa
       band. The band of 17 kDa seems to be useful to study the clinical
       evolution, for post-treatment control and also for epidemiologic
       purposes. (It has been identified in 7% of control sera from endemic
       areas.) Immunoblot could be a valuable tool in the diagnosis of visceral
       leishmaniasis, being more sensitive and specific than other serologic
       tests.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS  Adult  Animal
       Antibodies, Protozoan/BLOOD  *Antigens, Protozoan/CHEMISTRY
       Carbohydrates/CHEMISTRY/IMMUNOLOGY  Chagas Disease/IMMUNOLOGY  Child
       Cross Reactions  Epitopes/CHEMISTRY  Human
       Immunoblotting/*METHODS/STATISTICS & NUMER DATA  Leishmania
       infantum/*IMMUNOLOGY  Leishmaniasis,
       Visceral/COMPLICATIONS/*DIAGNOSIS/*IMMUNOLOGY  Molecular Weight
       Protozoan Proteins/CHEMISTRY/*IMMUNOLOGY  Sensitivity and Specificity
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

