       Document 0928
 DOCN  M9650928
 TI    Ex vivo evidence for PGE2 and LTB4 involvement in cutaneous
       leishmaniasis: relation with infection status and cytokine production.
 DT    9505
 AU    Milano S; Arcoleo F; Dieli M; D'Agostino R; De Nucci G; D'Agostino P;
       Cillari E; Institute of General Pathology, University of Palermo, Italy.
 SO    Parasitology. 1996 Jan;112 ( Pt 1):13-9. Unique Identifier : AIDSLINE
       MED/96155201
 AB    Ex vivo culture of spleen cells from BALB/c mice infected with 2 x 10(6)
       Leishmania major (L. major) promastigotes were cultured with
       ConcanavalinA (ConA) or leishmanial antigen (L. Ag) and tested for
       prostaglandin E2 (PGE2) and for leukotriene B4 (LTB4), in order to study
       their involvement in the evolution of cutaneous leishmaniasis and the
       connexion with lymphokine-mediated responses. The data were compared
       with those obtained in BALB/c mice protected against L. major by
       sublethal irradiation (550 rad; cured mice). In the unprotected BALB/c
       mice the levels of PGE2 that were responsible for the depression of
       interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF
       alpha) Th1-associated cytokines and for the relative increase in the
       interleukin-4 (IL-4) became higher and higher as the lesion progressed.
       On the contrary, the cured mice produced levels of PGE2 similar to
       normal uninfected controls, high levels of TNF alpha and IFN-gamma and
       low levels of IL-4. Elevated levels of LTB4 were detected in the early
       stage of infection in the unprotected mice compared to cured ones, a
       sign of more intense inflammation and a stimulus for the recruitment of
       inflammatory cells. The observation that exogenous LTB4 was able to
       enhance in vitro both Th1 cytokines in cured mice and Th2 cytokines in
       unprotected ones suggests that LTB4 could act in the recruitment of the
       T cells already committed to Th1 or Th2 phenotype.
 DE    Animal  Cells, Cultured  Dinoprostone/*METABOLISM  Female  Interferon
       Type II/METABOLISM  Interleukin-4/METABOLISM  Leishmania
       major/*IMMUNOLOGY  Leishmaniasis, Cutaneous/*IMMUNOLOGY/PATHOLOGY
       Leukotriene B4/*METABOLISM/PHARMACOLOGY  Mice  Mice, Inbred BALB C
       Spleen/CYTOLOGY/*METABOLISM  Support, Non-U.S. Gov't  Th1
       Cells/IMMUNOLOGY  Th2 Cells/IMMUNOLOGY  Tumor Necrosis Factor/METABOLISM
       Whole-Body Irradiation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

