       Document 0924
 DOCN  M9650924
 TI    Variable expression of major histocompatibility complex class II in the
       domestic cat.
 DT    9505
 AU    Hunt P; McConnell I; Grant CK; Else RW; Hopkins J; Department of
       Veterinary Pathology, University of Edinburgh,; Royal (Dick) School of
       Veterinary Studies, Summerhall.
 SO    Res Vet Sci. 1995 Nov;59(3):195-200. Unique Identifier : AIDSLINE
       MED/96155081
 AB    This paper describes the characterisation of six independently produced
       monoclonal antibodies (mAbs) specific for non-polymorphic determinants
       of feline major histocompatibility complex (MHC) class II. One mAb is an
       anti-sheep class II which cross-reacts with the cat and five have been
       produced in response to immunisation with purified feline
       immunodeficiency virus. Despite their independent source all the mAbs
       have identical reactivities, immunoprecipitating two complex groups of
       polypeptides of M(r) 33 to 36.000 (MHC class II alpha chains) and M(r)
       28 to 31,000 (MHC class II beta chains). Immunoblot analysis showed them
       to be beta chain-specific. One and two-dimensional electrophoresis
       revealed the complexity of feline class II mass and charge and implied
       the expression of multiple class II loci in the cat. Furthermore, it was
       demonstrated that distinct cell populations expressed a distinct range
       of class II variants. This suggesting either the differential expression
       or the distinct post-translational modification of lymphocytes from
       different sites. The mAbs have also been used for the detailed
       examination of the cellular distribution and tissue localisation of MHC
       class II in the cat.
 DE    Animal  Antibodies, Monoclonal/CHEMISTRY/IMMUNOLOGY  Cat
       Diseases/PATHOLOGY  Cats/BLOOD/*IMMUNOLOGY  Cell Line  Electrophoresis,
       Gel, Two-Dimensional  Flow Cytometry/VETERINARY  Genes,
       Immunoglobulin/GENETICS  Histocompatibility Antigens Class
       II/*ANALYSIS/GENETICS/  *IMMUNOLOGY  Immunoblotting/VETERINARY
       Immunodeficiency Virus, Feline/IMMUNOLOGY  Immunohistochemistry
       Leukemia, Lymphocytic/PATHOLOGY/VETERINARY  Lymph Nodes/IMMUNOLOGY
       Molecular Weight  Precipitin Tests/VETERINARY  Sheep  Skin/IMMUNOLOGY
       Spleen/IMMUNOLOGY  Support, Non-U.S. Gov't  Thymus Gland/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

