       Document 0923
 DOCN  M9650923
 TI    Occurrence of T lymphocytes in perivascular regions of the lung after
       intratracheal infection of swine with Pasteurella multocida.
 DT    9505
 AU    Berndt A; Muller G; Federal Institute for Health Protection of Consumers
       and; Veterinary Medicine, Jena Branch, Germany.
 SO    Vet Immunol Immunopathol. 1995 Nov;49(1-2):143-59. Unique Identifier :
       AIDSLINE MED/96158383
 AB    The defence mechanisms of the lung acting against airborne antigens
       (bacteria) are not yet fully understood. Therefore, we investigated
       cell-derived immunological processes in porcine pulmonary tissue. The
       incidence and distribution of leukocyte subpopulations in lungs, lung
       lymph nodes and tonsils from eight animals intratracheally infected with
       Pasteurella multocida (P.m.) type A wild strain and from four
       non-infected control animals were established by immunohistochemistry,
       using a panel of monoclonal antibodies (mAbs) against specific porcine
       leukocyte antigens: MHC Class II (MHC II), SWC1, SWC3a, CD2a, CD4a,
       CD8b. In the test animals, a broad layer of SWC1+ cells and SWC3a+ cells
       emerged in the subsinusoidal region of lung lymph nodes and in the
       bronchoalveolar spaces of the lung as early as 4 and 24 h after
       infection, and in the subsinusoidal regions of the lung lymph nodes only
       very few cells could be stained with the mAb against the MHC II antigen.
       We could not detect any MHC Class II+ cells in the bronchoalveolar
       spaces at this time of the investigation. In the course of the
       disappearance of the SWC1+ cells and SWC3+ cells, CD2a+, CD4a+ and, in
       lower numbers, CD8b+ T lymphocytes seemed to concentrate in the
       perivascular and, partially, the peribronchial regions of the lung 72 h
       after infection. By means of the double immunostaining method, we could
       demonstrate an accumulation of CD4a+ cells and CD8b+ cells after
       infection which lacked the SWC1 antigen, indicating that these cells
       were activated T lymphocytes. The same cell types (SWC1-/CD4a+ and
       SWC1-/CD8b+ cells) as well as CD8b-/CD4a+ cells could be observed in the
       interstitium of the lung 72 h after infection.
 DE    Animal  Antibodies, Monoclonal  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY/PATHOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY/PATHOLOGY  Immunohistochemistry  Lung/BLOOD
       SUPPLY/*IMMUNOLOGY/PATHOLOGY  Lymph Nodes/IMMUNOLOGY/PATHOLOGY
       *Pasteurella multocida  Pasteurella
       Infections/IMMUNOLOGY/PATHOLOGY/*VETERINARY  Swine  Swine
       Diseases/ETIOLOGY/*IMMUNOLOGY/PATHOLOGY  T-Lymphocyte
       Subsets/*IMMUNOLOGY/PATHOLOGY  Trachea  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

