       Document 0921
 DOCN  M9650921
 TI    CD8+ lymphocytes in bronchoalveolar lavage and blood: in vivo indicators
       of lung pathology caused by maedi-visna virus.
 DT    9505
 AU    Lujan L; Begara I; Collie DD; Watt NJ; Department of Veterinary
       Pathology, Royal (Dick) School of; Veterinary Studies, University of
       Edinburgh, Easter Bush, UK.
 SO    Vet Immunol Immunopathol. 1995 Nov;49(1-2):89-100. Unique Identifier :
       AIDSLINE MED/96158379
 AB    A study to determine the putative relationship between lymphocyte
       phenotypic alterations in bronchoalveolar lavage fluid and stage of lung
       pathology in maedi-visna infected sheep has been carried out. Twenty-one
       ewes (16 Texel and five Scottish blackface) naturally infected by
       maedi-visna virus and three Oxford controls were used. Animals were
       killed, lungs were removed, bronchoalveolar lavage was performed and
       pathological studies were completed. Blood samples were also obtained
       from 16 animals. Lymphocytes in both bronchoalveolar lavage and
       peripheral blood were labelled with monoclonal antibodies against the
       main T lymphocyte subsets (CD4, CD8, CD5 and gamma delta TCR) in order
       to perform flow cytometric studies. Three aspects of pathology were
       studied: lymphoid interstitial pneumonia, lymphoid follicular
       hyperplasia and smooth muscle hyperplasia. Percentages of CD4+, CD5+,
       gamma delta + T cells and the value for the CD4+ / CD8+ ratio in
       bronchoalveolar lavage of maedi-visna infected animals were
       significantly decreased (P < 0.05) when compared to controls, while
       percentages of CD8+ lymphocytes were increased in bronchoalveolar lavage
       of infected sheep and they were very close to being significant (P =
       0.07) when compared to controls. Lesions were evaluated and simple
       least-squares regression tests demonstrated that there were several
       significant correlations between various lymphocyte subsets and
       pathological parameters studied in this work. However, when a multiple
       regression test was applied to the data, it was observed that only the
       CD8+ T cell subset both in bronchoalveolar lavage and in blood was
       significantly correlated with severity of lung pathology. It is
       concluded that CD8+ lymphocytes are key cells in the development of the
       interstitial reaction and the lymphocytic alveolitis observed in
       maedi-visna infected ewes and that the CD8+ alveolitis is a parallel
       feature to the intensity of lung lesions. It is further suggested that
       the percentage of CD8+ lymphocytes in bronchoalveolar lavage and in
       blood may act as in vivo indicators of lung pathology in maedi-visna
       infected sheep.
 DE    Animal  Bronchoalveolar Lavage Fluid/*CYTOLOGY/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY/*PATHOLOGY  Female
       Lung/*IMMUNOLOGY/*PATHOLOGY  Lymphocyte Count  Lymphocyte
       Subsets/IMMUNOLOGY/PATHOLOGY  Phenotype  Pneumonia, Progressive
       Interstitial, of Sheep/*IMMUNOLOGY/  *PATHOLOGY  Sheep  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

