       Document 0492
 DOCN  M9640492
 TI    Specific decrease of Th1-like activity in mice with plasma cell tumors.
 DT    9604
 AU    Ruzek MC; Mathur A; Department of Microbiology, University of Minnesota,
       Minneapolis; 55455, USA.
 SO    Int Immunol. 1995 Jul;7(7):1029-35. Unique Identifier : AIDSLINE
       MED/96134426
 AB    Previously we examined the ability of the host's immune responses to
       regulate Ig production in an IgE-secreting murine plasma cell tumor
       (B53). In the present study we have examined the reverse phenomenon, in
       that we have investigated the effects of this and other plasma cell
       tumors on the immune responses of their hosts. We found that splenocytes
       from plasma cell tumor-bearing mice demonstrate decreased proliferation
       in response to polyclonal stimulation by either Con A or a combination
       of PMA and calcium ionophore (A23187). Fractionation of the splenocytes
       demonstrated that this reduction in proliferation was confined to CD4+ T
       cells and that the proliferation of CD8+ T cells was unaffected. In
       order to determine whether the down-modulatory effects of the tumor were
       confined to a particular CD4+ helper T cell subset, we examined the
       production of cytokines representing the Th1 subset (IL-2 and IFN-gamma)
       and the Th2 subset (IL-4 and IL-10) from stimulated splenocytes and from
       stimulated enriched splenic T cells. We found that both stimulated
       splenocytes and T cells from plasma cell tumor-bearing mice produced
       lower levels of the Th1 cytokines IL-2 and IFN-gamma compared with
       normal cultures, demonstrating that Th1-like responses are inhibited in
       the hosts of these tumors. However, no alterations in the production of
       the Th2 cytokines IL-4 and IL-10 were observed in these stimulated
       splenocyte or T cell cultures from the tumor-bearing mice.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Animal  Calcimycin/PHARMACOLOGY  Concanavalin A/PHARMACOLOGY
       Cytokines/BIOSYNTHESIS  CD4-Positive T-Lymphocytes/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female  *Lymphocyte
       Transformation  Mice  Mice, Inbred BALB C  Plasmacytoma/*IMMUNOLOGY
       Spleen/CYTOLOGY/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Tetradecanoylphorbol Acetate/PHARMACOLOGY  Th1
       Cells/*IMMUNOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

