       Document 0459
 DOCN  M9640459
 TI    Human immunodeficiency virus type 1 integrase stabilizes a linearized
       HIV-1 LTR plasmid in vivo.
 DT    9604
 AU    Faust EA; Acel A; Udashkin B; Wainberg MA; Lady Davis Institute for
       Medical Research, SMBD-Jewish General; Hospital, Montreal, Quebec,
       Canada.
 SO    Biochem Mol Biol Int. 1995 Jul;36(4):745-58. Unique Identifier :
       AIDSLINE MED/96057063
 AB    A mammalian expression vector designed for production of HIV-1 integrase
       was found to enhance the stability of a linear reporter plasmid in COS-7
       cells. The effect is strictly dependent on coexpression of the HIV-1 rev
       gene and on the inclusion of U3 and U5 portions of the HIV-1 LTR in the
       reporter plasmid. Integrase point mutations P109S and D116N drastically
       reduced stabilization whereas T115A and D64A had little or no effect.
       Immunoblot analysis revealed the presence of a 32-34kDa integrase
       protein in extracts of transfected COS-7 cells and of wild type and
       mutant integrase proteins at comparable levels. We conclude that
       integrase acts in trans in COS-7 cells, possibly by binding to the HIV-1
       LTR in the plasmid. This transfection system may be useful for studying
       factors that stabilize the HIV-1 DNA genome prior to its integration
       into the host cell chromosome.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Cercopithecus
       aethiops  DNA Nucleotidyltransferases/ANALYSIS/BIOSYNTHESIS/*METABOLISM
       *Genes, rev  Human  *HIV Long Terminal Repeat
       HIV-1/*ENZYMOLOGY/*GENETICS  Molecular Sequence Data  Molecular Weight
       Mutagenesis, Insertional  Mutagenesis, Site-Directed
       Oligodeoxyribonucleotides  Plasmids/*METABOLISM  Point Mutation
       Recombinant Proteins/ANALYSIS/BIOSYNTHESIS/METABOLISM  Restriction
       Mapping  Support, Non-U.S. Gov't  Transfection  Virus Integration
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

