       Document 0453
 DOCN  M9640453
 TI    Characterization of an antigen shared by human thymic epithelium and
       human T cell leukemia virus p19 Gag protein.
 DT    9604
 AU    Palker TJ; Singer KH; Vahlne A; Department of Medicine, Duke University
       Medical Center, Durham,; North Carolina, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;11(1):10-9.
       Unique Identifier : AIDSLINE MED/96130042
 AB    The molecular basis for cross-reactive antibody binding to human T cell
       leukemia virus type I (HTLV-I) p19 core protein and human thymic
       epithelium has been defined with two monoclonal antibodies (mAbs),
       12/1-2 and 13B12, raised to HTLV-I p19. The mAb 12/1-2 has previously
       been shown to react with HTLV-I p19, HTLV-II p22, and antigens of normal
       human thymic epithelium, placenta, and foreskin, whereas mAb 13B12 binds
       only to the carboxyl terminus of HTLV-I p19. In the present study, mAb
       12/1-2 bound to a subset of Triton X-100-insoluble intermediate
       filaments in human thymic epithelium also recognized by antikeratin
       antibodies AE1 and AE3. The mAb 12/1-2 also reacted in Western blot
       assays with proteins of 54, 46, and 40 kDa present in extracts of human
       thymic epithelium and with hexameric peptides containing overlapping
       sequences of HTLV-I p19 with the amino acids IPP (amino acids 117-119).
       In contrast, the HTLV-I-specific mAb 13B12 did not bind to human thymic
       epithelium and reacted with a single hexameric peptide containing the
       carboxy-terminal HTLV-I p19 sequence IPPPYV (amino acids 117-122).
       Binding of mAb 12/1-2 to thymic epithelium could be inhibited by
       adsorption with peptide SP-79 containing a C-terminal sequence (amino
       acids 112-125) of p19. The crossreactive IPP site is within a region of
       p19 that has been previously shown to be highly immunogenic in
       HTLV-I-infected individuals and that is also encoded by genes or mRNA of
       human cytokeratin 17, keratin 4, epidermal cytokeratin 2, and 50-kDa
       type I epidermal keratin. Thus, our studies define the sequence of a
       cross-reactive antigen on HTLV-I p19 that is also associated with
       keratin intermediate filaments from human thymic epithelium and other
       normal human tissues and that could serve as a focus of an autoimmune
       response during HTLV-I infection.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal  Blotting, Western
       Cells, Cultured  Child  Cloning, Molecular  Cross Reactions/IMMUNOLOGY
       Epithelium/IMMUNOLOGY  Epitopes/CHEMISTRY/IMMUNOLOGY  Fluorescent
       Antibody Technique  Gene Products, gag/CHEMISTRY/*IMMUNOLOGY  Human
       Hybridomas  HTLV-I/*IMMUNOLOGY  HTLV-I Antigens/CHEMISTRY/*IMMUNOLOGY
       Keratin/CHEMISTRY/IMMUNOLOGY  Male  Mice  Molecular Sequence Data
       Rabbits  Radioimmunoassay  Retroviridae Proteins,
       Oncogenic/CHEMISTRY/*IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Thymus Gland/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

