       Document 0450
 DOCN  M9640450
 TI    Human antibody variable region gene usage in HIV-1 infection.
 DT    9604
 AU    Wisnewski A; Cavacini L; Posner M; Department of Medicine, New England
       Deaconess Hospital, Boston; 02215, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;11(1):31-8.
       Unique Identifier : AIDSLINE MED/96130044
 AB    Human antibody variable region gene usage during human immunodeficiency
       virus type 1 (HIV-1) infection is examined in the following review, and
       several hypotheses are presented to account for the distinct patterns of
       antibody gene expression associated with infection. Evidence supporting
       qualitatively biased antibody gene expression has been derived from
       analysis of the human humoral immune response by isoelectric focusing
       (IEF) and serological and molecular studies of immunoglobulin (Ig) from
       different lymphoid compartments of HIV-1-infected patients. Preferential
       usage of heavy-chain variable region (VH) gene families 1 and 4 is
       supported by serological studies of serum Ig and molecular
       characterization of anti-HIV-1 human monoclonal antibodies derived from
       infected patients. Negative biases against VH3 family gene usage are
       detected by polymerase chain reaction (PCR) studies of peripheral blood
       lymphocytes from AIDS patients but not by combinatorial phage display
       library techniques. Biased antibody gene usage and expression during
       HIV-1 infection may be related to HIV-1 pathogenesis by limiting the
       available HIV-1 neutralizing repertoire. Further molecular
       characterization of anti-HIV-1 antibodies and in vivo expression of
       V-region genes during HIV-1 infection should provide important
       information regarding antibody gene expression and its relationship to
       HIV-1 pathogenesis.
 DE    Antibodies, Monoclonal  Gene Expression  *Genes, Immunoglobulin  Human
       HIV Antibodies/*IMMUNOLOGY  HIV Infections/ETIOLOGY/*IMMUNOLOGY
       HIV-1/GENETICS/*IMMUNOLOGY  IgG/IMMUNOLOGY  Immunoglobulin Variable
       Region/*GENETICS  Immunoglobulins, Heavy-Chain/*GENETICS  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

