       Document 0441
 DOCN  M9640441
 TI    Expression of CD69 after in vitro stimulation: a rapid method for
       quantitating impaired lymphocyte responses in HIV-infected individuals.
 DT    9604
 AU    Krowka JF; Cuevas B; Maron DC; Steimer KS; Ascher MS; Sheppard HW;
       California Department of Health Services, Berkeley 94704, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;11(1):95-104.
       Unique Identifier : AIDSLINE MED/96130053
 AB    A flow cytometric assay based on expression of the activation antigen
       CD69 was developed to analyze immunological responses of T cells from
       human immunodeficiency virus (HIV)-infected (HIV+) or HIV-seronegative
       (HIV-) donors after in vitro simulation by antigens and polyclonal
       activators. The levels of CD69 on freshly-isolated or unstimulated,
       cultured CD3+, CD4+, or CD8+ peripheral blood lymphocyte (PBL) subsets
       were low and did not differ greatly between HIV+ and HIV- donors. The
       frequencies of CD3+, CD4+, and CD8+ lymphocytes from HIV+ donors that
       expressed CD69 after culture with antigenic or mitogenic stimuli were
       significantly lower than in HIV- donors. Comparison of CD69 expression
       with [3H]thymidine incorporation revealed that both assays could detect
       lymphocyte responses to antigenic or mitogenic stimuli. The CD3+ PBL
       from HIV+ or HIV- donors did not show increased CD69 expression after
       culture with soluble or cross-linked recombinant envelope glycoprotein,
       gp120. The gp120, however, significantly inhibited CD69 expression in
       phytohemagglutinin-stimulated T cells in vitro and may also affect
       T-cell activation in vivo. These studies demonstrate the usefulness of
       this CD69 expression assay for the rapid assessment of defects in immune
       responses of phenotypically defined lymphocyte subsets in HIV+ patients
       and for testing the effects of agents that modulate immune activation.
 DE    Antigens, CD/*BIOSYNTHESIS  Antigens, CD3/IMMUNOLOGY  Antigens,
       Differentiation, T-Lymphocyte/*BIOSYNTHESIS  Cells, Cultured
       Comparative Study  CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA Replication  Flow Cytometry  Human  HIV
       Envelope Protein gp120/PHARMACOLOGY  HIV Infections/*IMMUNOLOGY  HIV
       Seropositivity/IMMUNOLOGY  Immunophenotyping  *Lymphocyte
       Transformation/DRUG EFFECTS  Male  Mitogens/PHARMACOLOGY  Prospective
       Studies  Recombinant Proteins  Support, U.S. Gov't, P.H.S.
       Thymidine/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

