       Document 0429
 DOCN  M9640429
 TI    Immunohistochemical studies on melanin associated antigen (MAA) induced
       experimental autoimmune anterior uveitis (EAAU).
 DT    9604
 AU    Kim MC; Kabeer NH; Tandhasetti MT; Kaplan HJ; Bora NS; Department of
       Ophthalmology and Visual Sciences, Washington; University School of
       Medicine, St Louis, MO 63110, USA.
 SO    Curr Eye Res. 1995 Aug;14(8):703-10. Unique Identifier : AIDSLINE
       MED/96002868
 AB    Experimental autoimmune anterior uveitis (EAAU), a model of uveitis
       induced by sensitization to melanin associated antigen (MAA) derived
       from the iris and ciliary body, closely resembles human acute anterior
       uveitis. The immunopathogenesis of EAAU was studied by
       immunohistochemical detection of immune cells and the expression of Ia,
       ICAM-1 and LFA-1 antigens. Male Lewis rats were immunized with bovine
       MAA, mixed with CFA and pertussis toxin in the hind foot pad. Animals
       were examined daily by slit-lamp biomicroscopy and serially sacrificed
       up to 30 days. Immunohistology of the enucleated eyes was performed with
       monoclonal antibodies W3/25 (CD4), OX-8 (CD8), ED2 (macrophage), OX-33
       (B cell), OX-6 (Ia), IA29 (ICAM-1) and WT.1 (LFA-1). During each stage
       of EAAU, CD4+ T cells predominated over both CD8+ T cells and
       macrophages in the uvea. Very few B cells were detected during each
       stage of EAAU. EAAU could not be induced by the adoptive transfer of
       sera obtained from immunized animals. Low levels of constitutive ICAM-1
       and Ia were observed. An increase in ICAM-1 expression was first noted
       on the epithelial cells of the uveal tract and RPE on day 9 post
       immunization and preceded LFA-1 and Ia upregulation by approximately 2
       days. The immunopathogenesis of EAAU appears to be linked to the
       presence of the CD4+ T cells.
 DE    Animal  Antibodies, Monoclonal  Antigens/*IMMUNOLOGY  Autoimmune
       Diseases/*IMMUNOLOGY/METABOLISM/PATHOLOGY  B-Lymphocytes/IMMUNOLOGY
       Cattle  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Histocompatibility Antigens Class
       II/BIOSYNTHESIS  Immunization  Immunoenzyme Techniques  Immunotherapy,
       Adoptive  Intercellular Adhesion Molecule-1/BIOSYNTHESIS  Lymphocyte
       Function-Associated Antigen-1/BIOSYNTHESIS  Macrophages/IMMUNOLOGY  Male
       Melanins/*IMMUNOLOGY  Rats  Rats, Inbred Lew  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Uveitis,
       Anterior/*IMMUNOLOGY/METABOLISM/PATHOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

