       Document 0428
 DOCN  M9640428
 TI    A novel function of islet-derived CD8+T cells in initiating and
       developing autoimmune insulin-dependent diabetes mellitus in non-obese
       diabetic (NOD) mice.
 DT    9604
 AU    Amano K; Yokono K; Hasegawa Y; Taki T; Tominaga Y; Yoneda R; Nagata M;
       Kasuga M; Second Department of Internal Medicine, Kobe University School
       of; Medicine, Japan.
 SO    Diabetes Res Clin Pract. 1995 Jun;28(3):161-72. Unique Identifier :
       AIDSLINE MED/96105588
 AB    Accumulated studies revealed that CD4+T cells were initially required
       for diabetes in NOD mice, whereas interaction of CD4+T/CD8+T cells is
       not fully understood. To address this question, we established
       islet-derived CD4+T cells and CD8+T cells from NOD mice. One NOD neonate
       that received CD4+T cells developed diabetes and insulitis with CD8+T
       cells. Administration of cyclophosphamide to non-diabetic recipients
       accelerated the development of diabetes, while none of the mice with
       anti-CD8 antibody did so. Similarly, it was observed that neonates that
       received islet-derived CD8+T cells developed diabetes and obvious
       insulitis mainly with CD4+T cells. Administration of anti-CD4 antibody
       with transfer of CD8+T cells inhibited insulitis. These results imply
       that CD8+T cells function as an initial element to recruit CD4+T cells
       to islets as well as a final effector.
 DE    Animal  Antibodies, Monoclonal  Antigens, CD4/IMMUNOLOGY/PHYSIOLOGY
       Cell Separation  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY/PATHOLOGY  Diabetes Mellitus,
       Insulin-Dependent/*IMMUNOLOGY  Flow Cytometry  Hybridomas  Islets of
       Langerhans/*IMMUNOLOGY/PATHOLOGY  Lymphocyte Transformation  Mice  Mice,
       Inbred NOD  Pancreatic Diseases/IMMUNOLOGY/PATHOLOGY  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

