       Document 0336
 DOCN  M9640336
 TI    Analysis of feline dual lymphocyte populations observed by flow
       cytometry.
 DT    9604
 AU    Walker C; Bao S; Canfield PJ; Department of Veterinary Pathology,
       University of Sydney, N.S.W.,; Australia.
 SO    Vet Immunol Immunopathol. 1995 Sep;48(1-2):11-25. Unique Identifier :
       AIDSLINE MED/96106999
 AB    Two discrete lymphocyte populations were observed commonly on flow
       cytometric analysis (FCM) of feline lymphocyte subsets. The identity of
       these populations as small and large lymphocytes was established by
       correlating data from FCM with that from peripheral blood films. Dual
       lymphocyte populations were more likely to be seen in feline
       immunodeficiency virus-positive (FIV(-)+ ve) cats but their occurrence
       was not affected by health status, age, gender or breed. FIV(-)+ ve cats
       had a significantly higher proportion of large lymphocytes than
       FIV-negative (FIV(-)- ve) cats. However, FIV(-)+ ve cats had
       significantly fewer small lymphocytes than FIV(-)- ve cats but similar
       numbers of large lymphocytes. Lymphocyte subset analysis revealed that
       small lymphocytes had a greater proportion of CD4+ cells than large
       lymphocytes, regardless of the FIV or health status of the cat. In
       FIV(-)- ve cats, small lymphocytes had a greater proportion of Pan T +
       lymphocytes than large lymphocytes, but the converse was seen in FIV(-)+
       ve cats. The proportion of CD8 + cells was higher in small lymphocytes
       than large lymphocytes in well FIV(-)- ve cats but this distinction was
       not seen in sick FIV(-)- ve cats or FIV(-)+ ve cats of any health
       status. Regardless of health status, FIV(-)+ ve cats had a lower
       absolute count of small lymphocytes which were T cells (due to lower
       numbers of both CD4 + and CD8 + cells) than FIV(-)- ve cats. The numbers
       of small B cells were similar for both FIV(-)+ ve and FIV(-)- ve cats.
       However, there were no differences between FIV(-)+ ve and FIV(-)- ve
       cats in the absolute values of any subset of the large lymphocytes,
       which suggested that FIV may affect only small lymphocytes.
       Statistically, the inclusion or exclusion of the large lymphocyte
       population for routine lymphocyte subset analysis did not affect the
       overall results. However, because there were significant differences in
       subset distribution between small and large lymphocytes, analysis of
       both groups should be included in studies examining the role of
       lymphocytes in disease.
 DE    Animal  Antigens/METABOLISM  Cat Diseases/IMMUNOLOGY  Cats  Female  Flow
       Cytometry  Health Status  Immunodeficiency Virus, Feline/IMMUNOLOGY
       Lentivirus Infections/IMMUNOLOGY/VETERINARY  Lymphocyte Count/VETERINARY
       Lymphocyte Subsets/*CLASSIFICATION/IMMUNOLOGY/VIROLOGY  Male
       Scattering, Radiation  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

