       Document 0334
 DOCN  M9640334
 TI    Effect of bovine leukemia virus infection on bovine peripheral blood
       monocyte responsiveness to lipopolysaccharide stimulation in vitro.
 DT    9604
 AU    Werling D; Sileghem M; Lutz H; Langhans W; Institute for Animal
       Sciences, Swiss Federal Institute of; Technology, ETH-Zentrum, Zurich,
       Switzerland.
 SO    Vet Immunol Immunopathol. 1995 Sep;48(1-2):77-88. Unique Identifier :
       AIDSLINE MED/96107004
 AB    The effects of bovine leukemia virus (BLV) infection on cytokine
       activity of bovine monocytes stimulated with Escherichia coli
       lipopolysaccharide (LPS) were examined. Compared to supernatants of
       LPS-stimulated monocytes from BLV-negative cows, supernatants from
       BLV-positive cows contained about four times more interleukin-1 beta
       (IL-1 beta) (as measured by an enzyme-linked immunosorbent assay (ELISA)
       for bovine IL-1 beta). Despite their higher IL-1 beta concentration,
       supernatants from BLV-positive cows stimulated proliferation of murine
       thymocytes in the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-biphenyl
       tetrazolium bromide) assay similar to supernatants from BLV-negative
       cows, but showed about 30% less IL-1 activity than supernatants from
       BLV-negative cows on the IL-1-dependent cell line LBRM-33 1 A-5, and
       about five times more tumor necrosis factor (TNF) activity on the
       TNF-sensitive murine fibroblast cell line L-929. These results
       demonstrate that BLV infection changes the cytokine response of bovine
       monocytes to LPS stimulation in vitro. The results are consistent with
       the assumption that BLV infection leads to the production and secretion
       of a soluble IL-1 inhibitor by LPS-stimulated peripheral blood
       monocytes.
 DE    Animal  Cattle  Cell-Free System/IMMUNOLOGY  Enzootic Bovine
       Leukosis/BLOOD/*IMMUNOLOGY  Female  Interleukin-1/BLOOD/PHARMACOLOGY
       Leukemia Virus, Bovine/*IMMUNOLOGY  Lipopolysaccharides/*PHARMACOLOGY
       Lymphocyte Transformation/DRUG EFFECTS  Monocytes/DRUG
       EFFECTS/*IMMUNOLOGY  T-Lymphocytes/IMMUNOLOGY  Tumor Necrosis
       Factor/IMMUNOLOGY/TOXICITY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

