       Document 0331
 DOCN  M9640331
 TI    [Interferon-alpha controls HPV infection in cervix epithelium]
 DT    9604
 AU    Labeit D; Labeit S; Berger M; Gallati H; Rosenberg R; Friese K;
       Universitatsfrauenklinik Mannheim.
 SO    Zentralbl Gynakol. 1995;117(11):566-77. Unique Identifier : AIDSLINE
       MED/96098816
 AB    Decreased immune response is necessary for a prolonged HPV-infection and
       allows HPV to be virulent as an oncogene. This study shows that
       HPV-infection in cervical epithelium is determined by the immune system
       and IFN-alpha can be shown to be a prognostic parameter for the
       HPV-infection. The cytokines (IFN-alpha, IFN-gamma, and TNF-alpha) from
       stimulated peripheral blood mononuclear cells (PBMC) were measured using
       monoclonal IFN-antibodies and ELISA test. To detect HPV-16, PCR (e6 and
       e7 areas) was used, followed by southern-blot of the PCR-products. In
       all of our patients (n = 139) no cytological change was observed in the
       cervical epithelium over a period of 3 years. Comparison was made
       between 3 groups: 1: controls (n = 89, HPV-pos. n = 6) 2: registered
       prostitutes without drug abuse (n = 30, HPV-pos. n = 6) and 3:
       HIV-infected, previous drug users (CDC II, n = 20, HPV-pos. n = 2).
       RESULTS: The stimulated IFN-alpha values are highest in the control
       collective (169 +/- 35 U/ml) and are significantly lower in the
       prostitutes (98 +/- 26 U/ml, p < 0.05) and in the HIV-infected group (49
       +/- 15 U/ml, p < 0.01). The difference between the latter groups being
       significant as well (p < 0.05). Dividing the controls into HPV-16
       positive and HPV-16 negative subgroups, the IFN-alpha values are
       significantly higher in HPV-16 negative group (193 +/- 48 U/ml) compared
       to HPV-16 positive group (38 +/- 3 U/ml, p < 0.05). Also in the
       collective of prostitutes and HIV infected patients there is a similar
       significant difference between the HPV-16 positive and HPV-16 negative
       patients (Prostitutes: HPV-16 negative = 94 +/- 21 U/ml, HPV-16 positive
       = 36 +/- 7 U/ml, p < 0.05; HIV-infected: HPV-16 negative = 35 +/- 13
       U/ml, HPV-16 positive = 13 +/- 3 U/ml).
 DE    Adult  Antiviral Agents/*BLOOD  AIDS-Related Opportunistic
       Infections/IMMUNOLOGY  Cervix Neoplasms/*IMMUNOLOGY  Cervix
       Uteri/VIROLOGY  English Abstract  Female  Human  Interferon Type
       II/BLOOD  Interferon-alpha/*BLOOD  Middle Age  Papillomavirus,
       Human/*IMMUNOLOGY  Papovaviridae Infections/*IMMUNOLOGY  Prostitution
       Support, Non-U.S. Gov't  Tumor Necrosis Factor/METABOLISM  Tumor Virus
       Infections/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

