       Document 0281
 DOCN  M9640281
 TI    Increased soluble tumor necrosis factor receptor expression and release
       by human immunodeficiency virus type 1 infection.
 DT    9604
 AU    Kalinkovich A; Geleziunas R; Kemper O; Belenki D; Wallach D; Wainberg
       MA; Bentwich Z; R. Ben-Ari Institute of Clinical Immunology, Kaplan
       Hospital,; Hebrew University Medical School, Rehovot, Israel.
 SO    J Interferon Cytokine Res. 1995 Sep;15(9):749-57. Unique Identifier :
       AIDSLINE MED/96097341
 AB    High levels of circulating soluble tumor necrosis factor receptors
       (sTNF-R) are associated with HIV-1 infection and disease. To understand
       better this association, we have investigated p55 and p75 TNF-R
       expression on peripheral blood mononuclear cell (PBMC) subsets and in
       the promonocytic cell line U937, with or without HIV infection. Using
       flow cytometry and monoclonal antibodies both to sTNF-R and to PBMC
       subsets, TNF-R were found to be expressed mostly by monocytes and in
       decreasing amounts and intensity in the following order: CD14+ cells >
       CD8+ cells > CD4+ cells. Expression of TNF-R was higher on cells
       obtained from HIV-infected than from noninfected subjects, and
       expression of p75 sTNF-R was much higher than that of p55 sTNF-R.
       Studying the U937 cells revealed that over 80% of the cells expressed
       both sTNF-R, but with greater fluorescence intensity in the HIV-1
       chronically infected cells (U-937-IIIB). Treatment of the cells with PMA
       caused an accelerated release into the medium of both sTNF-R, with a
       sharp decline in their cell surface expression. Basal levels of mRNA
       transcripts for p75 TNF-R were higher in the U-937-IIIB cells than in
       the uninfected cells, but p55 TNF-R mRNA was expressed only in the
       HIV-1-infected cells. These findings show that HIV-1 infection is
       accompanied by predominant elevation of p75 TNF-R surface expression on
       monocytes and CD8+ lymphocytes, and results in both increased message
       and expression of these receptors in monocytes.(ABSTRACT TRUNCATED AT
       250 WORDS)
 DE    Acquired Immunodeficiency Syndrome/*BLOOD  Biological Markers/BLOOD
       Cell Line  CD8-Positive T-Lymphocytes/*PATHOLOGY  Human  *HIV-1
       Leukocytes, Mononuclear/METABOLISM  Lymphocyte Count
       Monocytes/METABOLISM  Receptors, Tumor Necrosis
       Factor/*BIOSYNTHESIS/METABOLISM  RNA, Messenger/BIOSYNTHESIS  Solubility
       Support, Non-U.S. Gov't  Tetradecanoylphorbol Acetate/ANTAGONISTS &
       INHIB  Tumor Necrosis Factor/ANTAGONISTS & INHIB  Virus Replication/DRUG
       EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

