       Document 0277
 DOCN  M9640277
 TI    Cell cycling in HIV infection: analysis of in vivo activated
       lymphocytes.
 DT    9604
 AU    Mahalingam M; Pozniak A; McManus TJ; Vergani D; Peakman M; Department of
       Immunology, King's College School of Medicine and; Dentistry, London,
       UK.
 SO    Clin Exp Immunol. 1995 Dec;102(3):481-6. Unique Identifier : AIDSLINE
       MED/96128136
 AB    Infection with HIV results in increased circulating levels of T
       lymphocytes expressing phenotypic markers of immune activation. In the
       present study, using three-colour immunofluorescence, we examined the
       cell cycle status of these activated cells. Activated (HLA-DR+, CD25+
       and CD38+) CD4+ and CD8+ T lymphocytes in peripheral blood were analysed
       for DNA content in 15 HIV+ patients and 10 healthy age- and sex-matched
       control subjects. As expected, all HIV+ patients had elevated percentage
       levels of activated CD4+ HLA-DR+, CD4+ CD25+, CD8+ HLA-DR+, CD8+ CD25+
       and CD8+ CD38+ T lymphocytes compared with control subjects (P < 0.001
       for all). Percentage levels of CD4+ HLA-DR+ and CD8+ HLA-DR+T
       lymphocytes in the 'proliferative' (S-G2M) phase of the cell cycle were
       also higher in the HIV+ patients compared with controls (P < 0.001 for
       both). The percentage levels of proliferative CD4+ CD25+, CD8+ CD25+ and
       CD8+ CD38+ lymphocytes were, however, similar in HIV+ patients and
       controls, indicating that the proliferative fraction of cells in vivo
       was confined to the HLA-DR+ subset and absent from the CD25+ and CD38+
       populations. Four HIV+ patients had grossly elevated levels of CD8+
       lymphocytes which were CD38+ (> 95%) and confined to the pre-G0-G1 phase
       of the cell cycle, suggesting these may be cells committed to apoptosis.
       These observations indicate an increase in the proliferative capacity of
       HLA-DR+ T lymphocytes in HIV infection in vivo. The reduced DNA content
       in other populations (e.g. CD38+ CD8+ lymphocytes) of some patients with
       advanced HIV disease suggests that these cells are apoptotic. Thus our
       results define both proliferative and apoptotic processes as a spectrum
       of activation-related events in HIV infection.
 DE    Adult  Antigens, Differentiation/ANALYSIS  Cell Cycle  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female
       Human  HIV Infections/*IMMUNOLOGY  HLA-DR Antigens/ANALYSIS  *Lymphocyte
       Transformation  Male  Nucleosidases/ANALYSIS  Support, Non-U.S. Gov't
       T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

