       Document 0274
 DOCN  M9640274
 TI    T-cell repertoire of normal, rejected, and pathological corneas:
       phenotype and function.
 DT    9604
 AU    Wackenheim-Urlacher A; Kantelip B; Falkenrodt A; Piqot X; Tongio MM;
       Montard M; Delbosc B; Department d'Ophtalmologie, CHU Jean Minjoz,
       Besancon, France.
 SO    Cornea. 1995 Sep;14(5):450-6. Unique Identifier : AIDSLINE MED/96037081
 AB    The specific immune mechanisms of corneal graft rejection are not
       completely understood. Recently, the technique of growing T-cell lines
       from rejected allografts using recombinant IL-2 has enabled the cells
       involved in allograft rejection to be recognized. In the present study,
       this method was applied for the extraction and propagation of T
       lymphocytes from rejected, normal, and diseased corneas. T-cell lines
       could successfully be grown from rejected and normal corneas, but not
       from corneas with keratoconus or pseudophakic bullous keratopathy. The
       phenotypic repertoire of the grown cells was studied by FACS scan
       analysis. Rejected corneas were invaded by a mixture of activated CD4+
       and CD8+ T-cell lines, with one population being predominant. In normal
       corneas only activated CD8+ cells with cytotoxic function were cultured.
       No cells were obtained from diseased corneas. The in vitro function of
       cell lines was assessed by primed lymphocyte testing. The present study
       shows that the technique of propagating invading T-cell lines from organ
       grafts can be applied to human corneas, offering a new approach to
       understanding the immunological mechanisms occurring inside this immune
       tissue.
 DE    Antibodies, Monoclonal  Antigens, CD/ANALYSIS  Cell Line  Cell
       Separation  Cells, Cultured  Cornea/*CYTOLOGY/PATHOLOGY  Corneal
       Diseases/*PATHOLOGY  Corneal Transplantation  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Flow
       Cytometry  Graft Rejection/*PATHOLOGY  Human  *Immunophenotyping
       Receptors, Antigen, T-Cell/ANALYSIS
       T-Lymphocytes/*CLASSIFICATION/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

