       Document 0258
 DOCN  M9640258
 TI    Molecular characterization of human monoclonal antibodies specific for
       several HIV proteins: analysis of the VH3 family expression.
 DT    9604
 AU    David D; Goossens D; Desgranges C; Theze J; Zouali M; Unite
       d'Immunogenetique Cellulaire, Institut Pasteur, Paris,; France.
 SO    Immunol Lett. 1995 Jul-Aug;47(1-2):107-12. Unique Identifier : AIDSLINE
       GENBANK/Z35126
 AB    We have analyzed the heavy-chain variable (VH) region genes expressed by
       a panel of human monoclonal antibodies derived from an immunized
       volunteer, an AIDS patient and seropositive asymptomatic donors, and
       specific for HIV-1 env, pol and gag gene products. The third
       complementarity-determining regions show a high complexity with
       unconventional gene recombination events. Most of the VH genes utilized
       are also frequently encountered in other immune responses. Their
       sequences are, in general, typical of an antigen-driven immune response.
       Molecular mechanisms that generate high-affinity antibodies are then
       effective during HIV infection. Remarkably, VH3 family, which dominates
       the human antibody repertoire, is barely encountered among anti-HIV
       antibodies.
 DE    Antibodies, Monoclonal/*CHEMISTRY/GENETICS  Antibody
       Specificity/*GENETICS  Base Sequence  Gene Products, env/IMMUNOLOGY
       Gene Products, gag/IMMUNOLOGY  Gene Products, pol/IMMUNOLOGY  Genes,
       Reiterated  Human  HIV Antigens/GENETICS/*IMMUNOLOGY  Immunoglobulin
       Variable Region/ANALYSIS/*GENETICS  Immunoglobulins,
       Heavy-Chain/ANALYSIS/*GENETICS  Molecular Sequence Data  Mutation
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

