       Document 0250
 DOCN  M9640250
 TI    Design, synthesis, and characterization of HIV-1 enhancer-binding
       polypeptides derived from bacteriophage 434 repressor.
 DT    9604
 AU    Stadler K; Liu N; Trotman L; Hiltpold A; Caderas G; Klauser S; Hehlgans
       T; Gutte B; Biochemical Institute of Zurich University, Switzerland.
 SO    Int J Pept Protein Res. 1995 Sep-Oct;46(3-4):333-40. Unique Identifier :
       AIDSLINE MED/96122184
 AB    We have designed and synthesized HIV-1 enhancer-binding polypeptides
       that were derived from bacteriophage 434 repressor. These peptides were
       39-54 residues long and contained either the recognition helix or the
       entire helix-turn-helix motif of the DNA-binding domain of 434
       repressor. The dissociation constant of the complex formed between the
       standard peptide (R42) and a synthetic 70-bp HIV enhancer DNA was ca.
       10(-8) M. The specificity of the interaction of R42 with the two HIV
       enhancers was demonstrated by competitive band shift assays, stepwise
       displacement of the p50 subunit of transcription factor NF-kappa B from
       its two HIV enhancer binding sites, and DNase I footprinting; R42 seemed
       to protect best the two TTTCC sequences of the HIV enhancers against
       digestion by DNase I. R42 analogues with mutated recognition helix had
       lower DNA binding specificity. It remains to be investigated whether our
       artificial HIV enhancer-binding polypeptides are active in vivo.
 DE    Amino Acid Sequence  Bacteriophages/*CHEMISTRY  Base Sequence  Binding
       Sites  Chromatography, Affinity  Circular Dichroism  Deoxyribonuclease I
       *Drug Design  DNA Footprinting  DNA-Binding Proteins/*CHEMICAL
       SYNTHESIS/METABOLISM  DNA, Viral/*CHEMISTRY/METABOLISM  *HIV Enhancer
       HIV-1/*GENETICS  Molecular Sequence Data  Repressor
       Proteins/*CHEMISTRY/METABOLISM  Spectrophotometry, Ultraviolet  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

