       Document 0242
 DOCN  M9640242
 TI    Anti-CD3-induced changes in protein kinase C isozymes expression in
       human CD4+ and CD8+ T lymphocytes.
 DT    9604
 AU    Harris W; Gollapudi S; Gupta S; Division of Basic and Clinical
       Immunology, University of; California, Irvine 92717, USA.
 SO    J Clin Immunol. 1995 Sep;15(5):232-41. Unique Identifier : AIDSLINE
       MED/96108691
 AB    In order to determine whether there is a differential expression and
       activation of PKC isozymes between CD4+ and CD8+ T cells, peripheral
       blood mononuclear cells were stimulated with anti-CD3 monoclonal
       antibody (moAb) for various time intervals and the expression of
       calcium-dependent PKC isozymes (alpha, beta, gamma) and
       calcium-independent PKC isozymes (delta, epsilon, zeta) was analyzed
       with dual color flow cytometry, using anti-PKC isozyme antibodies and
       anti-CD4 or anti-CD8 antibodies. The basal fluorescence intensity of all
       PKC isozymes was comparable between CD4+ T cells and CD8+ T cells.
       Following activation with anti-CD3 moAb a marked increase in the
       fluorescence intensity of all PKC isozymes in both CD4+ and CD8+ T
       cells, albeit to a different extent and with different kinetics was
       observed. Among all PKC isozymes studied, the least striking changes
       were observed in PKC zeta isozyme and the most striking changes were
       observed in PKC-epsilon isozyme. Laser-based confocal microscopic
       studies confirmed that the increase in fluorescence intensity of PKC
       isozymes following anti-CD3 moAb stimulation, as measured by flow
       cytometry was accompanied by the translocation of PKC isozymes from
       cytosol to the plasma membrane. This study demonstrates a differential
       effect of anti-CD3 moAb on the expression of PKC isozymes between CD4+
       and CD8+ T cells and suggests that flow cytometry can be used to study
       the translocation of PKC isozymes from cytosol to the plasma membrane.
 DE    Antibodies, Monoclonal/*PHARMACOLOGY  Antigens, CD3/*IMMUNOLOGY
       CD4-Positive T-Lymphocytes/*ENZYMOLOGY  CD8-Positive
       T-Lymphocytes/*ENZYMOLOGY  Flow Cytometry  Fluorescence  Human
       Isoenzymes/*BLOOD  Lymphocyte Transformation  Microscopy, Confocal
       Protein Kinase C/*BLOOD  Subcellular Fractions/ENZYMOLOGY  T-Lymphocyte
       Subsets  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

