       Document 0233
 DOCN  M9640233
 TI    Use of intrinsic and extrinsic helper epitopes for in vivo induction of
       anti-hepatitis C virus cytotoxic T lymphocytes (CTL) with CTL epitope
       peptide vaccines.
 DT    9604
 AU    Shirai M; Chen M; Arichi T; Masaki T; Nishioka M; Newman M; Nakazawa T;
       Feinstone SM; Berzofsky JA; Department of Microbiology, Yamaguchi
       University School of; Medicine, Japan.
 SO    J Infect Dis. 1996 Jan;173(1):24-31. Unique Identifier : AIDSLINE
       MED/96132459
 AB    The induction of virus-specific cytotoxic T lymphocytes (CTL) is an
       important part of vaccine strategy. CTL induction in vivo by two
       hepatitis C virus (HCV) peptides containing CTL epitopes, one from the
       NS5 region (P17) and one from the core (C7), was compared. P17 required
       covalent attachment of a helper peptide (PCLUS3 containing a cluster of
       epitopes from the human immunodeficiency virus envelope protein),
       whereas C7 did not. However, the minimal decapeptide of C7, C7A10, alone
       did not induce CTL. The helper cells induced by PCLUS3-17 or by C7 were
       shown to be CD4+ and to produce interleukin-2 (IL-2). Thus, help can be
       supplied by a natural helper epitope intrinsic to the CTL peptide, as in
       C7, or by attaching a helper epitope from another protein, as in the
       case of P17. The cluster peptides may be useful promiscuous helper
       peptides for a variety of CTL epitopes from diverse pathogens.
 DE    Amino Acid Sequence  Animal  Comparative Study  Cytotoxicity,
       Immunologic  Epitopes/*IMMUNOLOGY  Gene Products,
       env/CHEMISTRY/IMMUNOLOGY  Hepatitis C Antibodies/*IMMUNOLOGY  Hepatitis
       C Viruses/*IMMUNOLOGY  Hepatitis, Viral, Animal/IMMUNOLOGY  HIV-1
       Immunization  Interleukin-2/BIOSYNTHESIS  Mice  Mice, Inbred BALB C
       Molecular Sequence Data  Peptide Fragments/IMMUNOLOGY  Protein
       Precursors/CHEMISTRY/IMMUNOLOGY  Spleen/CYTOLOGY/IMMUNOLOGY  Support,
       Non-U.S. Gov't  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  T-Lymphocytes,
       Helper-Inducer/*IMMUNOLOGY  Vaccines, Synthetic/IMMUNOLOGY  Viral Core
       Proteins/CHEMISTRY/IMMUNOLOGY  Viral Hepatitis Vaccines/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

