       Document 0223
 DOCN  M9640223
 TI    Laboratory diagnosis of infection status in infants perinatally exposed
       to human immunodeficiency virus type 1.
 DT    9604
 AU    Paul MO; Tetali S; Lesser ML; Abrams EJ; Wang XP; Kowalski R; Bamji M;
       Napolitano B; Gulick L; Bakshi S; Pahwa S; Department of Pediatrics,
       North Shore University Hospital-Cornell; University Medical College,
       Manhasset, NY 11030, USA.
 SO    J Infect Dis. 1996 Jan;173(1):68-76. Unique Identifier : AIDSLINE
       MED/96132465
 AB    Accurate and timely diagnosis of infection status in infants born to
       women infected with human immunodeficiency virus (HIV) is of paramount
       importance. The comparative accuracy of five diagnostic decision rules
       was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected)
       based on laboratory testing during the first 6 months of life.
       Diagnostic rules A and B, which required single blood samples analyzed
       by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR,
       and p24 antigen detection (rule B) were more prone to incorrect
       diagnoses than were rules requiring 2 blood samples analyzed by a single
       assay (rule C) or combinations of culture and PCR (rules D and E). Rule
       D, which used PCR as the initial test, established the most useful
       algorithm: a positive PCR result followed by a positive culture in the
       second sample confirmed infected status, while two consecutive negative
       PCR results reconfirmed as negative at 6 months of age established
       uninfected status.
 DE    Blood/VIROLOGY  Cohort Studies  *Disease Transmission, Vertical  DNA,
       Viral/ANALYSIS  False Negative Reactions  Female  Health Status  Human
       HIV Antibodies/ANALYSIS  HIV Core Protein p24/ANALYSIS  HIV
       Infections/*DIAGNOSIS/TRANSMISSION  *HIV-1/GENETICS/IMMUNOLOGY/ISOLATION
       & PURIF  Infant  Infant, Newborn  Maternal-Fetal Exchange  Polymerase
       Chain Reaction  Predictive Value of Tests  Pregnancy  *Pregnancy
       Complications, Infectious  Prospective Studies  Reproducibility of
       Results  Sensitivity and Specificity  Support, U.S. Gov't, P.H.S.  Virus
       Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

