       Document 0222
 DOCN  M9640222
 TI    Herpes simplex virus protein targets for CD4 and CD8 lymphocyte
       cytotoxicity in cultured epidermal keratinocytes treated with
       interferon-gamma.
 DT    9604
 AU    Mikloska Z; Kesson AM; Penfold ME; Cunningham AL; Virology Department,
       Westmead Hospital, Australia.
 SO    J Infect Dis. 1996 Jan;173(1):7-17. Unique Identifier : AIDSLINE
       MED/96132457
 AB    In early recurrent herpetic lesions, CD4 T lymphocytes are the
       predominant infiltrating cells, and keratinocytes expressing major
       histocompatibility complex (MHC) class II antigens, induced by
       interferon-gamma (IFN-gamma), are the major site of herpes simplex virus
       (HSV) replication. IFN-gamma pretreatment of human keratinocytes in
       vitro reduced MHC class I antigen down-regulation by HSV-1 infection and
       induced expression of HLA-DR that was unaltered by subsequent HSV-1
       infection. Incubation of these infected keratinocytes with
       phosphonoacetic acid (PAA) almost completely inhibited expression of
       four major HSV glycoproteins, although expression of early proteins was
       not affected. Weak CD8 T lymphocyte cytotoxicity against
       IFN-gamma-stimulated, HLA-DR-expressing HSV-1-infected keratinocytes was
       consistently directed to the immediate early/early proteins (all 9
       patients tested) but against late proteins to a lesser degree (4/9
       patients). However, CD4 T lymphocyte cytotoxicity was much greater and
       directed predominantly against late HSV-1 glycoproteins (all 9 subjects
       tested) in these cells.
 DE    Antiviral Agents/*PHARMACOLOGY  Cells, Cultured  Cytotoxicity,
       Immunologic  CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Down-Regulation (Physiology)
       Fibroblasts/DRUG EFFECTS/IMMUNOLOGY/VIROLOGY  Flow Cytometry
       Herpesvirus 1, Human/*PHYSIOLOGY  Histocompatibility Antigens Class
       I/BIOSYNTHESIS  Histocompatibility Antigens Class II/BIOSYNTHESIS  Human
       Immediate-Early Proteins/BIOSYNTHESIS  Interferon-gamma,
       Recombinant/*PHARMACOLOGY  Keratinocytes/DRUG
       EFFECTS/*IMMUNOLOGY/VIROLOGY  Killer Cells, Natural/IMMUNOLOGY
       Phosphonoacetic Acid/PHARMACOLOGY  Reverse Transcriptase
       Inhibitors/PHARMACOLOGY  Skin/CYTOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/METABOLISM  Viral Envelope
       Proteins/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

