       Document 0145
 DOCN  M9640145
 TI    Differential inhibition of reverse transcriptase and cellular DNA
       polymerase-alpha activities by lignans isolated from Chinese herbs,
       Phyllanthus myrtifolius Moon, and tannins from Lonicera japonica Thunb
       and Castanopsis hystrix.
 DT    9604
 AU    Chang CW; Lin MT; Lee SS; Liu KC; Hsu FL; Lin JY; Institute of
       Biochemistry, College of Medicine, National Taiwan; University, Taiwan.
 SO    Antiviral Res. 1995 Aug;27(4):367-74. Unique Identifier : AIDSLINE
       MED/96049784
 AB    Two lignans, phyllamycin B and retrojusticidin B isolated from
       Phyllanthus myrtifolius Moon have been demonstrated to have a strong
       inhibitory effect on human immunodeficiency virus-1 reverse
       transcriptase activity (HIV-1 RT), but much less inhibitory effect on
       human DNA polymerase-alpha (HDNAP-alpha) activity. Fifty percent
       inhibitory concentrations of phyllamycin B and retrojusticidin B were
       determined to be 3.5 and 5.5 microM for HIV-1 RT, and 289 and 989 microM
       for HDNAP-alpha, respectively. The mode of inhibition was found to be
       non-competitive inhibition with respect to template-primer and
       triphosphate substrate. Several tannins such as caffeoylquinates (CQs)
       isolated from Lonicera japonica Thunb, galloylquinates (GQs) and
       galloylshikimates (GSs) purified from Castanopsis hystrix were shown to
       have a much less selective inhibitory effect on HIV-1 RT.
 DE    Drugs, Chinese Herbal/CHEMISTRY/*PHARMACOLOGY  DNA Polymerase
       II/*ANTAGONISTS & INHIB  Human  Kinetics  Lignans/ISOLATION &
       PURIF/*PHARMACOLOGY  Reverse Transcriptase Inhibitors/ISOLATION &
       PURIF/*PHARMACOLOGY  RNA-Directed DNA Polymerase/*DRUG EFFECTS
       Structure-Activity Relationship  Support, Non-U.S. Gov't
       Tannins/ISOLATION & PURIF/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

