       Document 0123
 DOCN  M9640123
 TI    Re-evaluation of skin-explant model in graft-versus-host disease
       prediction.
 DT    9604
 AU    Sahin S; Akoglu TF; Gurbuz O; Ergun T; Kullu S; Sav A; Ahiskali R;
       Tuzuner N; Dogusoy G; Department of Hematology and Immunology, Marmara
       University; Medical School, Istanbul, Turkiye.
 SO    Clin Transplant. 1995 Oct;9(5):370-4. Unique Identifier : AIDSLINE
       MED/96081141
 AB    An in vitro skin explant model has been proposed for prediction of graft
       versus host disease and reported to be highly sensitive and specific for
       this purpose. In this study we have re-evaluated this model in both HLA
       full-matched BMT recipient-donor sibling pairs and also in HLA one
       haplotype-matched parent-children pairs. All assessments were made
       blindly by 3 independent observers. The predictive value of the test for
       the occurrence of clinical GvHD in 14 BMT patients was found to be less
       sensitive than reported previously (correlation coefficients were
       +0.019, +0.067 and -0.061 between clinical GvHD and in vitro primed
       allogeneic, primed mixed and unprimed allogeneic settings, indicating
       poor correlation). False positive and false negative results were high
       and there were also significant discrepancies between three blind
       observations in the grading of skin changes. Weighted kappa analysis
       revealed that there were fair correlations between the 3 observers (K =
       0.25). These results indicated that the skin explant model is an
       unpredictable test system and there are great problems in
       standardization of the method.
 DE    Adult  Bone Marrow Transplantation/*IMMUNOLOGY  Child  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Graft
       vs Host Disease/*IMMUNOLOGY/PREVENTION & CONTROL  *Histocompatibility
       Testing  Human  HLA Antigens/IMMUNOLOGY  Leukemia/IMMUNOLOGY/THERAPY
       Lymphoma, Non-Hodgkin's/IMMUNOLOGY/THERAPY  Predictive Value of Tests
       Risk Factors  Skin/*IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

