       Document 0105
 DOCN  M9640105
 TI    A highly sensitive polymerase chain reaction method reveals the
       ubiquitous presence of maternal cells in human umbilical cord blood.
 DT    9604
 AU    Petit T; Gluckman E; Carosella E; Brossard Y; Brison O; Socie G;
       Laboratoire de Genetique Oncologique, URA 1967 CNRS, Institut; Gustave
       Roussy, Villejuif, France.
 SO    Exp Hematol. 1995 Dec;23(14):1601-5. Unique Identifier : AIDSLINE
       MED/96130296
 AB    Umbilical cord blood is considered an alternate source of hematopoietic
       stem cells in bone marrow transplantation. However, its use might be
       hampered by contamination of neonatal blood with maternal cells, which
       could contribute unacceptably to graft-vs.-host disease (GVHD) after
       transplant. In a previous study (Socie et al., Blood 83:340, 1994), we
       used polymerase chain reaction (PCR) amplification of minisatellite
       sequences (sensitivity 1-0.1%) to address the question of this
       contamination. In a single case among 47 analyzed, we were able to
       detect a maternal-specific allele in the cord blood sample. We have now
       studied the same cord samples using a highly sensitive, allele-specific
       PCR amplification method. A maternal allele could be discriminated from
       neonate alleles in 10 cases and maternal cells were detected in all 10
       cord blood samples. These cells amounted to 10(-4) to 10(-5) of cord
       blood nucleated cells. In three cases, cord blood separated cell
       subpopulations could be analyzed and were found to contain maternal
       cells at about the same level. The presence of maternal cells at such a
       low level in cord blood samples probably would have no effect on GVHD in
       a clinical setting of transplantation but raises interesting questions
       in terms of materno-fetal immune tolerance and transmission of viruses
       (in particular human immunodeficiency virus) from infected mother to
       child.
 DE    Alleles  Base Sequence  Cell Separation/*METHODS
       DNA/*ANALYSIS/CHEMISTRY  Female  Fetal Blood/*CYTOLOGY  Graft vs Host
       Disease/PREVENTION & CONTROL  Hematopoietic Stem Cell Transplantation
       Hematopoietic Stem Cells/*CYTOLOGY  Human  Iodide Peroxidase/*GENETICS
       Molecular Sequence Data  *Polymerase Chain Reaction  Pregnancy  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

