       Document 0104
 DOCN  M9640104
 TI    The Gag polypeptides of the Drosophila 1731 retrotransposon are
       associated to virus-like particles and to nuclei.
 DT    9604
 AU    Haoudi A; Kim MH; Champion S; Best-Belpomme M; Maisonhaute C;
       Laboratoire de Genetique Cellulaire et Moleculaire, UA 1135 CNRS,;
       Universite Pierre and Marie Curie, Paris, France.
 SO    FEBS Lett. 1995 Dec 11;377(1):67-72. Unique Identifier : AIDSLINE
       MED/96130323
 AB    1731 is a Drosophila melanogaster retrotransposon whose nucleotide
       sequence shows a proviral architecture with two long terminal repeats
       (LTRs) framing two internal Open Reading Frames (ORFs). The pol ORF2 of
       this mobile genetic element was demonstrated to code for an active
       Reverse Transcriptase (RT) and the ORF1 is expected to code for the
       structural Gag proteins of the virus-like particles (VLP). Using
       specific anti-Gag antibodies, we have characterized the 1731 Gag
       polypeptides expressed either in vitro or in Kc Drosophila melanogaster
       cultured cells. Together with the 1731 RT, the largest, likely
       post-translationaly-modified Gag polypeptides are gathered into
       cytoplasmic virus-like particles. Moreover and consistent with the
       nuclear localization signal present in the Gag sequence, we observed
       that a short 1731 Gag polypeptide is associated to the cell nuclei.
 DE    Amino Acid Sequence  Animal  Blotting, Western  Cell Nucleus/*CHEMISTRY
       Cells, Cultured  Drosophila melanogaster/*GENETICS  Gene Products,
       gag/*ANALYSIS/GENETICS  Immunosorbent Techniques  Molecular Sequence
       Data  Open Reading Frames  Repetitive Sequences, Nucleic Acid
       Restriction Mapping  *Retrotransposons  RNA-Directed DNA
       Polymerase/GENETICS  Support, Non-U.S. Gov't  Virion/*CHEMISTRY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

