       Document 0098
 DOCN  M9640098
 TI    Utilization of used human immunodeficiency virus ELISA microplates &
       surplus reagents for HIV antibody testing.
 DT    9604
 AU    Makhija SK; Dhanvijay AG; Pathak AA; Saoji AM; AIDS Surveillance Centre
       & Regional STD Reference Centre,; Department of Microbiology, Government
       Medical College &; Hospital, Nagpur.
 SO    Indian J Med Res. 1995 Oct;102:159-61. Unique Identifier : AIDSLINE
       MED/96108007
 AB    Used non-competitive enzyme-linked immunosorbent assay (ELISA)
       microplates were washed and reused to test samples and positive and
       negative controls, utilising the surplus reagents provided with the kit,
       which otherwise would have been discarded as useless after the entire
       960 test kit had been utilized. These surplus reagents could be used for
       additional 220 tests over and above the recommended 960 tests per kit. A
       total of 839 unknown serum samples, 54 negative controls and 36 positive
       controls were tested using both washed and fresh (new) ELISA plates
       simultaneously. The optical density (OD) value of the control sera was
       within the prescribed limits in both the methods and 15 samples were
       found to be positive for HIV antibodies by the fresh plates whereas the
       washed plates showed 18 samples to be positive for HIV antibodies. None
       of the samples positive by fresh plates were negative by washed plates.
 DE    Cost Control  Enzyme-Linked Immunosorbent Assay/*INSTRUMENTATION
       Equipment Reuse/*ECONOMICS  Female  Human  HIV Antibodies/*BLOOD
       Indicators and Reagents  Male  Microchemistry  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

