       Document 0097
 DOCN  M9640097
 TI    Preferred nucleotide sequence at the integration target site of human
       T-cell leukemia virus type I from patients with adult T-cell leukemia.
 DT    9604
 AU    Chou KS; Okayama A; Su IJ; Lee TH; Essex M; Department of Cancer
       Biology, Harvard School of Public Health,; Boston, MA 02115, USA.
 SO    Int J Cancer. 1996 Jan 3;65(1):20-4. Unique Identifier : AIDSLINE
       MED/96125762
 AB    Human T-cell leukemia virus type I (HTLV-I) is etiologically associated
       with adult T-cell leukemia/lymphoma (ATL). We cloned and sequenced host
       DNA adjacent to the long terminal repeats of HTLV-I from uncultured
       leukemic cells of 4 ATL patients. The region flanking the provirus was
       generally A/T-rich (60-64% A/T), and a nucleotide composition bias was
       noticed when sequences within 25 bp on both sides of the integration
       target site were analyzed. In the 6-bp direct repeat, both end positions
       are preferentially occupied by G/C, whereas the middle positions are
       preferentially occupied by A/T. Furthermore, AA or TT dinucleotides are
       frequently present on each side adjacent to the center of the direct
       repeat. Our finding suggests preferential integration target sites of
       HTLV-I in the host genome. Further study is warranted to determine
       whether each of the target sequence preference is a general property of
       HTLV-I integration or may be associated with the leukemogenesis of ATL.
 DE    Adult  Base Composition  Base Sequence  DNA Primers/CHEMISTRY  Human
       HTLV-I/*GENETICS  HTLV-I Infections/*GENETICS  Leukemia,
       T-Cell/*MICROBIOLOGY  Molecular Sequence Data  Repetitive Sequences,
       Nucleic Acid  Support, U.S. Gov't, P.H.S.  *Virus Integration  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

