       Document 0096
 DOCN  M9640096
 TI    Herpesvirus-like DNA sequences detected in endemic, classic, iatrogenic
       and epidemic Kaposi's sarcoma (KS) biopsies.
 DT    9604
 AU    Buonaguro FM; Tornesello ML; Beth-Giraldo E; Hatzakis A; Mueller N;
       Downing R; Biryamwaho B; Sempala SD; Giraldo G; Istituto Nazionale
       Tumori Fond. Pascale, Naples, Italy.
 SO    Int J Cancer. 1996 Jan 3;65(1):25-8. Unique Identifier : AIDSLINE
       MED/96125763
 AB    The identification of Kaposi's sarcoma (KS) clusters in sub-equatorial
       Africa (endemic KS, AKS) and the high frequency of KS in sexually
       transmitted AIDS (epidemic KS, EKS), have previously suggested a role
       for infectious agents in the etiopathogenesis of KS. The recent
       identification of herpesvirus (HHV)-like DNA sequences in one case of
       EKS and their detection in > 90% of all tested EKS, prompted us to
       determine the prevalence of these viral sequences in all types of KS,
       such as AKS, EKS, classic KS (CKS) and iatrogenic KS (IKS). The presence
       of herpesvirus(HHV)-like DNA sequences has been examined in 61 KS skin
       tumors obtained from Greece, Italy, USA, Uganda and Kenya. All KS types
       (100%) were positive by polymerase chain reaction (PCR) and
       Southern-blot analysis, while 5 out of 6 (83%) and 4 out of 7 (57%)
       uninvolved autologous skin biopsies from AKS and CKS patients,
       respectively, were positive for HHV-like sequences. All samples from
       non-KS patients were negative, i.e. 17 human biopsies from healthy
       individuals or patients affected by other pathologies, 5 human cell
       lines and 15 peripheral blood mononuclear cells (PBMC) from HIV-positive
       subjects. These results suggest that HHV-like sequences play a major
       role in the pathogenesis of this neoplasm.
 DE    DNA, Viral/ANALYSIS  Herpesviridae/*GENETICS  Human  Polymerase Chain
       Reaction  Sarcoma, Kaposi's/ETIOLOGY/*MICROBIOLOGY  Skin/MICROBIOLOGY
       Skin Neoplasms/ETIOLOGY/*MICROBIOLOGY  Support, Non-U.S. Gov't  Tumor
       Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

