       Document 0094
 DOCN  M9640094
 TI    Immunohistochemistry in histoid leprosy.
 DT    9604
 AU    Kontochristopoulos GJ; Aroni K; Panteleos DN; Tosca AD; Hansen's Disease
       Center, General Hospital of Western Attica,; Athens, Greece.
 SO    Int J Dermatol. 1995 Nov;34(11):777-81. Unique Identifier : AIDSLINE
       MED/96128698
 AB    BACKGROUND. Histoid leprosy is a rare form of multibacillary leprosy as
       the result of secondary or even primary resistance to dapsone. The
       etiopathogenesis has not been clarified up to now. METHODS. An
       immunohistochemical study was carried out for the expression of various
       markers on epidermal and dermal cell populations using sections of
       frozen skin specimens from 5 patients with histoid leprosy as compared
       to specimens from 7 tuberculoid and 7 lepromatous patients. RESULTS.
       Dendritic epidermal cells, identified by monoclonal antibodies against
       CD1, HLA-DR, CD45, and CD36, were found reduced in histoid leprosy as
       compared to both tuberculoid and lepromatous groups. A gradual reduction
       of keratinocytic HLA-DR expression from tuberculoid to lepromatous to
       histoid leprosy was observed. The pattern of CD36, CD4, and CD8
       expression of lymphomonocytic cells in the dermis of histoid lesions was
       similar to that of tuberculoid leprosy, but without the formation of an
       organized granuloma. CD45+ cells as well as activated lymphocytic cells,
       expressed by the activation immunophenotype (CD1, HLA-DR, CD25, CD71,
       EGF-R) were found frequently in all groups. CONCLUSIONS. The in situ
       immunohistochemical findings support a modified hypersensitivity
       reaction of the cellular type that results in an inhibition of the
       lesional expansion, but not in the destruction of the bacilli within the
       histoid lesion.
 DE    Aged  Antigens, CD1/ANALYSIS  Antigens, CD36/ANALYSIS  Antigens,
       CD45/ANALYSIS  CD4-CD8 Ratio  Dapsone/THERAPEUTIC USE  Dendritic
       Cells/IMMUNOLOGY/PATHOLOGY  Drug Resistance  Female  Human  HLA-DR
       Antigens/ANALYSIS  Immunohistochemistry
       Keratinocytes/IMMUNOLOGY/PATHOLOGY  Leprosy/DRUG
       THERAPY/*IMMUNOLOGY/*PATHOLOGY  Lymphocyte Subsets  Male  Middle Age
       Receptors, Epidermal Growth Factor-Urogastrone/ANALYSIS
       Skin/IMMUNOLOGY/PATHOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

