       Document 0090
 DOCN  M9640090
 TI    Tissue diagnosis of intestinal microsporidiosis using a fluorescent
       stain with Uvitex 2B.
 DT    9604
 AU    Franzen C; Muller A; Salzberger B; Fatkenheuer G; Eidt S; Mahrle G;
       Diehl V; Schrappe M; Department of Internal Medicine I, University of
       Cologne,; Germany.
 SO    J Clin Pathol. 1995 Nov;48(11):1009-10. Unique Identifier : AIDSLINE
       MED/96138182
 AB    AIMS--To detect intestinal microsporidiosis in paraffin wax embedded
       biopsy specimens using a fluorescence technique incorporating optical
       brighteners. METHODS--Eight HIV infected patients with confirmed
       intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with
       Encephalitozoon intestinalis and one with Encephalitozoon cuniculi
       infection) and 10 without infection were studied. Tissue sections of
       paraffin wax embedded duodenal biopsy specimens were stained with 1%
       Uvitex 2B, coded and analysed independently by two investigators.
       RESULTS--In all eight cases with confirmed intestinal microsporidian
       infection, spores could be detected easily in tissue sections using the
       fluorescence technique. Spores or other elements consistent with
       microsporidiosis were not found in the 10 patients without infection.
       CONCLUSION--Staining of tissue sections from paraffin wax embedded
       intestinal biopsy specimens with stains incorporating Uvitex 2B is a
       rapid and easy technique for the diagnosis of intestinal
       microsporidiosis.
 DE    Animal  AIDS-Related Opportunistic Infections/*DIAGNOSIS
       Duodenum/PARASITOLOGY  Fluorescent Dyes  Human  Intestinal Diseases,
       Parasitic/*DIAGNOSIS  Microspora Infections/*DIAGNOSIS
       Microsporida/*ISOLATION & PURIF  Staining/METHODS  CLINICAL TRIAL
       CONTROLLED CLINICAL TRIAL  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

