       Document 0084
 DOCN  M9640084
 TI    Structure and expression of murine CD30 and its role in cytokine
       production.
 DT    9604
 AU    Bowen MA; Lee RK; Miragliotta G; Nam SY; Podack ER; Bristol-Myers Squibb
       Pharmaceutical Research Institute, Seattle,; WA 98121, USA.
 SO    J Immunol. 1996 Jan 15;156(2):442-9. Unique Identifier : AIDSLINE
       GENBANK/U25416
 AB    Murine CD30 cDNA predicts a protein of 498 amino acids with homology to
       the TNF receptor family of proteins characterized by repeated
       cysteine-rich motifs in the extracellular domain. Murine CD30, although
       homologous to human CD30, has a 90 amino acid gap in an extracellular
       region that appears to be duplicated in human CD30. Murine CD30 cDNA was
       shown to be functional through the production of a soluble murine Ig
       fusion protein (CD30-Ig) that was active in binding to cells that
       expressed CD30 ligand. CD30-Ig also served as an immunogen for the
       production of hamster anti-mouse CD30 mAbs, which recognized both CD30
       expressed by murine lymphocytes and CD30 expressed by cells transfected
       with murine CD30 cDNA. CD30 mRNA is highly expressed in the thymus and
       in activated spleen cells, but not in other tissues tested. In
       anti-CD3-activated spleen cells, CD30 ligand is expressed primarily by
       CD4+ T cells, with peak expression at days 1 and 2, whereas CD30 is
       expressed primarily by CD8+ T cells, with peak expression on days 4 and
       5. Stimulation of CD30 by plate-bound anti-CD30 directly signaled for
       IL-5 but not IFN-gamma production by CD30+ CTL lines. These studies
       demonstrate that CD30 directs cytokine secretion and suggest that CD30
       signaling may be pivotal in the pattern of cytokine production by T
       cells.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/IMMUNOLOGY/ISOLATION
       & PURIF  Antigens, CD30/BIOSYNTHESIS/CHEMISTRY/IMMUNOLOGY/*PHYSIOLOGY
       Base Sequence  Cloning, Molecular  Comparative Study  CD8-Positive
       T-Lymphocytes/*SECRETION  DNA, Complementary/GENETICS  Hamsters  Human
       Interleukin-5/BIOSYNTHESIS  Leukemia P388/PATHOLOGY
       Lymphokines/*SECRETION  Lymphoma, T-Cell/PATHOLOGY  Membrane
       Glycoproteins/BIOSYNTHESIS  Mice  Mice, Inbred BALB C  Molecular
       Sequence Data  Organ Specificity  Recombinant Fusion
       Proteins/BIOSYNTHESIS  Sequence Homology, Amino Acid  Species
       Specificity  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Transfection  Tumor Cells, Cultured  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

