       Document 0073
 DOCN  M9640073
 TI    Adjuvant-free hsp70 fusion protein system elicits humoral and cellular
       immune responses to HIV-1 p24.
 DT    9604
 AU    Suzue K; Young RA; Whitehead Institute for Biomedical Research,
       Cambridge, MA 02142,; USA.
 SO    J Immunol. 1996 Jan 15;156(2):873-9. Unique Identifier : AIDSLINE
       MED/96133020
 AB    Heat shock proteins are major targets of the immune response to
       bacterial and parasitic pathogens. Mycobacterium tuberculosis hsp70 is
       an especially powerful Ag containing multiple B and T cell epitopes. We
       investigated whether M. tuberculosis hsp70 can be used as an
       adjuvant-free carrier to stimulate the humoral and cellular immune
       response to an accompanying protein. A recombinant hsp70 protein
       expression vector was developed that permits the production of any
       protein fused to the amino terminus of mycobacterial hsp70. We found
       that a recombinant HIV p24-hsp70 fusion protein produced with this
       vector elicited both humoral and cellular immune responses against p24
       in mice when administered in saline in the absence of adjuvant. Covalent
       linkage of hsp70 to p24 was essential to elicit immune responses to p24
       under these conditions. The anti-p24 IgG1 Abs induced in
       p24-hsp70-immunized mice persisted at high levels for more than 1 yr
       after immunization. These results demonstrate that the antigenic
       properties of M. tuberculosis hsp70 can be exploited to enhance the
       humoral and cellular immune response to an attached protein.
 DE    Animal  Antibody Specificity  Antigens, Bacterial/GENETICS/*IMMUNOLOGY
       AIDS Vaccines/*IMMUNOLOGY  Base Sequence  Chimeric Proteins/*IMMUNOLOGY
       Female  Genetic Vectors  Heat-Shock Proteins 70/GENETICS/*IMMUNOLOGY
       HIV Antibodies/*BIOSYNTHESIS/IMMUNOLOGY  HIV Core Protein
       p24/GENETICS/*IMMUNOLOGY  IgG/BIOSYNTHESIS/IMMUNOLOGY  Immunity,
       Cellular  Mice  Mice, Inbred BALB C  Molecular Sequence Data  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  Vaccines, Synthetic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

