       Document 0053
 DOCN  M9640053
 TI    Sera from HIV-1 infected individuals in all stages of disease
       preferentially recognize the V3 loop of the prototypic macrophage-tropic
       glycoprotein gp120 ADA.
 DT    9604
 AU    Ebenbichler C; McNearney T; Stoiber H; Most J; Zangerle R; Vogetseder W;
       Patsch JR; Ratner L; Dierich MP; Institut fur Hygiene und
       Ludwig-Boltzmann-Institut fur; AIDS-Forschung, Innsbruck, Austria.
 SO    Mol Immunol. 1995 Oct;32(14-15):1039-45. Unique Identifier : AIDSLINE
       MED/96128272
 AB    The outer membrane glycoprotein gp120 and the transmembrane glycoprotein
       gp41 are predominant targets of the humoral immune response to infection
       by human immunodeficiency virus type 1. The third hypervariable region
       (V3 loop) is the principal neutralizing domain and is the primary target
       of neutralizing antibodies directed against the envelope proteins of
       HIV-1. The V3 loop is also the major determinant for HIV-1 cell-specific
       tropism. To further characterize the humoral immune response directed
       against the gp120 envelope proteins, we expressed two prototypic gp120
       envelope proteins (LAI/HXB2 and ADA) and chimeric gp120 envelope
       proteins in stable transfected Drosophila melanogaster Schneider 2
       cells. Sera from four infected adults over the course of infection
       [McNearney et al. (1992) Proc. natn. Acad. Sci. U.S.A. 89, p. 10,242]
       were assayed for reactivity with the respective envelope proteins. Sera
       obtained at early stages preferentially recognized the gp120 envelope
       protein ADA, whereas in later stages of infection the sera showed
       diminished reactivity with both gp120 LAI/HXB2 and gp120 ADA. Chimeric
       envelope proteins revealed that the humoral response was directed
       primarily against the V3 loop of gp120 ADA. Furthermore, 22 sera from
       HIV-1 infected individuals in different stages of the disease were
       tested. Reactivity of sera with the gp120 envelope protein ADA was
       seven-fold higher than with the gp120 envelope protein LAI/HXB2. Our
       results suggest that the humoral immune response is preferentially
       elicited against the V3 loop of the prototypic macrophage-tropic gp120
       envelope protein ADA.
 DE    Adult  Amino Acid Sequence  Antigen-Antibody Reactions  Chimeric
       Proteins/BLOOD/IMMUNOLOGY  Cross-Sectional Studies  Human  HIV Envelope
       Protein gp120/*IMMUNOLOGY  HIV Infections/BLOOD/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Immune Sera/CHEMISTRY  Immunodominant
       Epitopes/*IMMUNOLOGY  Longitudinal Studies
       Macrophages/*IMMUNOLOGY/VIROLOGY  Molecular Sequence Data  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

