       Document 0015
 DOCN  M9640015
 TI    DNA inoculation induces cross clade anti-HIV-1 responses.
 DT    9604
 AU    Wang B; Boyer J; Srikantan V; Ugen K; Agadjanian M; Merva M; Gilbert L;
       Dang K; McCallus D; Moelling K; et al; Apollon Inc., Malvern,
       Pennsylvania, USA.
 SO    Ann N Y Acad Sci. 1995 Nov 27;772:186-97. Unique Identifier : AIDSLINE
       MED/96135359
 AB    Nucleic acid or DNA immunization represents a novel approach to vaccine
       and immune therapeutic development. The direct injection of expression
       cassettes into a living host results in in vivo gene expression and
       immune activation. In the case of HIV-1 it has been shown by our
       laboratory that facilitated injection mimicks aspects of live attenuated
       vaccines and that both humoral and cellular responses can be induced
       upon injection of a nucleic acid sequence directly into a host target
       tissue. Antisera from HIV-1 plasmid expression cassette-immunized
       animals contain anti-HIV envelope glycoprotein immune responses. The
       antiserum neutralizes HIV-1 infection and inhibits cell to cell
       infection in vitro. Cellular immune responses have also been evaluated.
       We observed both T cell proliferation and isotype switching consistent
       with the production of relevant T helper immune responses in immunized
       animals. Furthermore it was demonstrated that CTL lysis of relevant
       env-expressing targets was similarly induced. These studies further
       define the importance of evaluating this new technology for vaccine and
       immune therapeutic development for HIV-1 as well as for other human
       viral pathogens.
 DE    Animal  Antibody Specificity  AIDS Vaccines/*IMMUNOLOGY  *DNA,
       Recombinant  Gene Products, env/GENETICS/*IMMUNOLOGY  *Genetic Vectors
       Human  HIV Antibodies/*BIOSYNTHESIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Lymphocyte Transformation  Macaca
       fascicularis/IMMUNOLOGY  Mice  Neutralization Tests  Protein
       Precursors/GENETICS/*IMMUNOLOGY  Recombinant Fusion
       Proteins/GENETICS/*IMMUNOLOGY  Repetitive Sequences, Nucleic Acid
       Sarcoma Viruses, Avian/GENETICS  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Th1 Cells/IMMUNOLOGY  Vaccines, Synthetic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

