       Document 0014
 DOCN  M9640014
 TI    Cytotoxic T lymphocyte and helper T cell responses following HIV
       polynucleotide vaccination.
 DT    9604
 AU    Shiver JW; Perry HC; Davies ME; Freed DC; Liu MA; Department of Virus
       and Cell Biology, Merck Research; Laboratories, West Point, Pennsylvania
       19486, USA.
 SO    Ann N Y Acad Sci. 1995 Nov 27;772:198-208. Unique Identifier : AIDSLINE
       MED/96135360
 AB    Expression vectors encoding either HIV-1 gp160/rev, gp120, or rev alone
       were used for direct vaccination of mice and nonhuman primates. Each
       vaccine elicited long-lived (> 7 months) helper T cell responses in mice
       and monkeys as measured by in vitro proliferation of splenocytes
       following recombinant antigen treatment. Cytokine assays of the cell
       supernatants showed that approximately 100-fold more gamma-interferon
       than IL-4 was secreted during culture indicating that these vaccines
       elicited TH1-like responses. CD8+ CTL activities were also observed both
       in mice and rhesus. The gp120 and gp160/rev vaccines elicited
       antigen-specific antibodies, although these responses were more variable
       and lower magnitude for gp160/rev, and gp120 DNA-vaccinated African
       green monkeys had moderate levels of neutralizing antibodies. No
       antibodies were found against rev (an intracellular protein) with either
       rev vaccine. Similar antibody titers were obtained for gp120 by either
       intramuscular or intradermal injection although T cell responses were
       generally lower by intradermal route. These results indicate that DNA
       vaccines may provide a powerful means to elicit cellular and humoral
       immune responses against HIV.
 DE    Animal  AIDS Vaccines/*IMMUNOLOGY  Cercopithecus aethiops/IMMUNOLOGY
       *DNA, Recombinant  Gene Products, env/GENETICS/*IMMUNOLOGY  Gene
       Products, rev/GENETICS/*IMMUNOLOGY  Genetic Vectors  HIV
       Antibodies/*BIOSYNTHESIS/IMMUNOLOGY  HIV Envelope Protein
       gp120/GENETICS/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Macaca mulatta/IMMUNOLOGY
       Mice  Mice, Inbred BALB C  Protein Precursors/GENETICS/*IMMUNOLOGY
       Recombinant Fusion Proteins/*IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY
       Vaccines, Synthetic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

