       Document 0718
 DOCN  M9630718
 TI    Kaposi's sarcoma-associated herpesvirus infects endothelial and spindle
       cells.
 DT    9603
 AU    Boshoff C; Schulz TF; Kennedy MM; Graham AK; Fisher C; Thomas A; McGee
       JO; Weiss RA; O'Leary JJ; Virology Laboratory, Royal Marsden Hospital,
       London, UK.
 SO    Nat Med. 1995 Dec;1(12):1274-8. Unique Identifier : AIDSLINE
       MED/96083627
 AB    Kaposi's sarcoma (KS), a vascular tumour that contains characteristic
       spindle cells forming slit-like spaces, may have an infectious
       aetiology. Recently, sequences of a new human herpesvirus, KSHV/HHV-8,
       have been identified in both HIV-associated and classical KS. We sought
       to identify the target cell of this virus in KS tumour tissue. Using PCR
       in situ hybridization (PCR-ISH) we show that KSHV/HHV-8 is present in
       the flat endothelial cells lining vascular spaces of KS lesions as well
       as in typical KS spindle cells. These findings show that KSHV/HHV-8 is
       present in the cell types thought to represent neoplastic cells in these
       lesions.
 DE    Adolescence  Adult  Aged  Aged, 80 and over  AIDS-Related Opportunistic
       Infections/PATHOLOGY/*VIROLOGY  Base Sequence  Endothelium  Female
       *Herpesviridae  Herpesviridae Infections/*VIROLOGY  Human  In Situ
       Hybridization  Male  Middle Age  Molecular Sequence Data  Nevus, Spindle
       Cell/*VIROLOGY  Polymerase Chain Reaction  Sarcoma,
       Kaposi's/PATHOLOGY/*VIROLOGY  Skin Neoplasms/PATHOLOGY/*VIROLOGY
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

