       Document 0716
 DOCN  M9630716
 TI    In vivo fate of HIV-1-infected T cells: quantitative analysis of the
       transition to stable latency.
 DT    9603
 AU    Chun TW; Finzi D; Margolick J; Chadwick K; Schwartz D; Siliciano RF;
       Department of Medicine, Johns Hopkins University School of; Medicine,
       Baltimore, Maryland 21205, USA.
 SO    Nat Med. 1995 Dec;1(12):1284-90. Unique Identifier : AIDSLINE
       MED/96083629
 AB    Although it is presumed that the integration of HIV-1 into the genome of
       infected CD4+ T lymphocytes allows viral persistence, there has been
       little direct evidence that CD4+ T cells with integrated provirus
       function as a latent reservoir for HIV-1 in infected individuals. Using
       resting CD4+ T-cell populations of extremely high purity and a novel
       assay that selectively and unambiguously detects integrated HIV-1, we
       show that resting CD4+ T cells harbouring integrated provirus are
       present in some infected individuals. However, these cells do not
       accumulate within the circulating pool of resting CD4+ T cells in the
       early stages of HIV-1 infection and do not accumulate even after
       prolonged periods in long-term survivors of HIV-1 infection. These
       results suggest that because of viral cytopathic effects and/or host
       effector mechanisms, productively infected CD4+ T cells do not generally
       survive for long enough to revert to a resting memory state in vivo.
 DE    Base Sequence  Cell Separation  CD4-Positive T-Lymphocytes/*VIROLOGY
       DNA Primers  DNA, Viral/*ANALYSIS  Human  HIV Infections/BLOOD/*VIROLOGY
       HIV-1/*GENETICS/ISOLATION & PURIF  Molecular Sequence Data  Polymerase
       Chain Reaction  Proviruses/*GENETICS  Support, U.S. Gov't, P.H.S.  Virus
       Integration  Virus Latency  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

