       Document 0714
 DOCN  M9630714
 TI    Dissecting protein:protein interactions between transcription factors
       with an RNA aptamer.
 DT    9603
 AU    Tian Y; Adya N; Wagner S; Giam CZ; Green MR; Ellington AD; Department of
       Chemistry, Indiana University, Bloomington 47405,; USA.
 SO    RNA. 1995 May;1(3):317-26. Unique Identifier : AIDSLINE MED/96079963
 AB    Nucleic acid aptamers isolated from random sequence pools have generally
       proven useful at inhibiting the interactions of nucleic acid binding
       proteins with their cognate nucleic acids. In order to develop reagents
       that could also be used to study protein:protein interactions, we have
       used in vitro selection to search for RNA aptamers that could interact
       with the transactivating protein Tax from human T-cell leukemia virus.
       Tax does not normally bind to nucleic acids, but instead stimulates
       transcription by interacting with a variety of cellular transcription
       factors, including the cyclic AMP-response element binding protein
       (CREB), NF-kappa B, and the serum response factor (SRF). Starting from a
       pool of greater than 10(13) different RNAs with a core of 120 random
       sequence positions, RNAs were selected for their ability to be
       co-retained on nitrocellulose filters with Tax. After five cycles of
       selection and amplification, a single nucleic acid species remained.
       This aptamer was found to bind Tax with high affinity and specificity,
       and could disrupt complex formation between Tax and NF-kappa B, but not
       with SRF. The differential effects of our aptamer probe on
       protein:protein interactions suggest a model for how the transcription
       factor binding sites on the surface of the Tax protein are organized.
       This model is consistent with data from a variety of other studies.
 DE    Base Sequence  Binding Sites  Binding, Competitive  DNA-Binding Protein,
       Cyclic AMP-Responsive/METABOLISM  DNA-Binding Proteins/METABOLISM  Gene
       Products, tax/ANTAGONISTS & INHIB/*METABOLISM  Molecular Sequence Data
       Nuclear Proteins/METABOLISM  NF-kappa B/METABOLISM  Protein Binding
       RNA/ANTAGONISTS & INHIB/*METABOLISM  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, Non-P.H.S.  Transcription Factors/*METABOLISM  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

