       Document 0707
 DOCN  M9630707
 TI    Differences in the interleukin-2 (IL-2) receptor system in human and
       mouse: alpha chain is required for formation of the functional mouse
       IL-2 receptor.
 DT    9603
 AU    Nemoto T; Takeshita T; Ishii N; Kondo M; Higuchi M; Satomi S; Nakamura
       M; Mori S; Sugamura K; Department of Microbiology, Tohoku University
       School of Medicine,; Sendai, Japan.
 SO    Eur J Immunol. 1995 Nov;25(11):3001-5. Unique Identifier : AIDSLINE
       MED/96085167
 AB    Reconstitution with mouse interleukin-2 (IL-2) receptor subunits
       demonstrated that the mouse IL-2 receptor complex was different from the
       human complex in the alpha chain requirement for the functional mouse
       receptor complex. The heterotrimeric complex of the mouse exogenous
       alpha and beta chains and the endogenous gamma chain on mouse lymphoid
       BW5147 cells showed the ability to bind IL-2 with high affinity,
       resulting in IL-2-induced tyrosine phosphorylation of a cytosolic
       tyrosine kinase, JAK3, which is involved in IL-2-dependent signals.
       Exogenous introduction of the beta chain with the endogenous gamma
       chain, however, could neither confer appreciable IL-2 binding nor
       IL-2-induced signal transduction on BW5147 cells, unlike the human beta
       gamma heterodimer. Mouse spleen CD8+ cells, not having the alpha chain
       initially, showed IL-2-dependent cell proliferation only when expression
       of the alpha chain was induced. Collectively, these results illustrate
       that the functional mouse IL-2 receptor complex necessarily includes the
       alpha chain, and that the regulation of CD8+ T cell growth during immune
       reaction depends upon alpha chain expression.
 DE    Animal  Antibodies, Monoclonal/IMMUNOLOGY  Cell Line  Comparative Study
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female  Human
       Interleukin-2/METABOLISM  Lymphocyte Transformation/IMMUNOLOGY  Mice
       Mice, Inbred BALB C  Protein-Tyrosine Kinase/IMMUNOLOGY  Rats  Rats,
       Wistar  Receptors, Interleukin-2/BIOSYNTHESIS/*IMMUNOLOGY/*METABOLISM
       Support, Non-U.S. Gov't  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

