       Document 0678
 DOCN  M9630678
 TI    Down-regulation of Epstein-Barr virus nuclear antigen 1 in
       Reed-Sternberg cells of Hodgkin's disease.
 DT    9603
 AU    Khan G; Naase MA; Department of Pathology, Tufts University School of
       Medicine,; Boston, MA 02111, USA.
 SO    J Clin Pathol. 1995 Sep;48(9):845-8. Unique Identifier : AIDSLINE
       MED/96096274
 AB    AIMS--To demonstrate Epstein-Barr virus (EBV) encoded nuclear antigen 1
       (EBNA-1) gene expression in EBV associated disorders using a new
       monoclonal antibody (1H4-1) on routinely processed tissues. METHODS--The
       pressure cooker antigen retrieval method was used for the
       immunohistochemical demonstration of EBNA-1 gene expression in formalin
       fixed, EBV positive tissues from Hodgkin's disease, infectious
       mononucleosis, HIV associated non-Hodgkin's lymphomas, post-transplant
       lymphomas, and undifferentiated nasopharyngeal carcinoma (NPC). EBV
       encoded EBNA-2, latent membrane protein 1 (LMP-1) and BZLF-1 gene
       expression was also examined using commercially available monoclonal
       antibodies. RESULTS--Of the 34 EBER in situ hybridisation positive cases
       of Hodgkin's disease examined, none expressed EBNA-1 in the
       Reed-Sternberg cells. These cells were nevertheless strongly LMP-1
       positive in all cases. Strong EBNA-1 staining was seen in all cases of
       EBER positive HIV associated non-Hodgkin's lymphoma (five of five),
       nasopharyngeal carcinoma (five of five), infectious mononucleosis (three
       of three), and post-transplant lymphoma (one of one). These cases also
       expressed LMP-1, EBNA-2 and BZLF-1, but at differing levels.
       CONCLUSION--The pressure cooker antigen retrieval procedure is a
       sensitive and reliable adjunct to immunohistochemistry, especially with
       antibodies which are otherwise ineffective on routinely processed
       tissues. The EBNA-1 gene is not expressed at detectable levels in the
       malignant cells of Hodgkin's disease, but is consistently expressed in
       other EBV associated disorders. This finding has important implications
       for the role of EBNA-1 in the biology of EBV.
 DE    Antibodies, Monoclonal  Antigens, Viral/GENETICS/*METABOLISM
       *Down-Regulation (Physiology)  DNA-Binding Proteins/GENETICS/*METABOLISM
       Herpesvirus 4, Human/*IMMUNOLOGY/ISOLATION & PURIF  Hodgkin's
       Disease/IMMUNOLOGY/*VIROLOGY  Human  Immunoenzyme Techniques  In Situ
       Hybridization  Reed-Sternberg Cells/IMMUNOLOGY/*VIROLOGY  Support,
       Non-U.S. Gov't  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

