       Document 0675
 DOCN  M9630675
 TI    Epidermal expression of 65 and 72 kd heat shock proteins in psoriasis
       and AIDS-associated psoriasiform dermatitis.
 DT    9603
 AU    Puig L; Fernandez-Figueras MT; Ferrandiz C; Ribera M; de Moragas JM;
       Department of Dermatology, Hospital de la Santa Creu i Sant Pau,;
       Barcelona, Spain.
 SO    J Am Acad Dermatol. 1995 Dec;33(6):985-9. Unique Identifier : AIDSLINE
       MED/96095113
 AB    BACKGROUND: Psoriasiform dermatitis is common in patients with AIDS. The
       expression of heat shock proteins by keratinocytes has been postulated
       to be a significant factor in the physiopathology of psoriasis and might
       be subject to modulation in HIV-infected patients. OBJECTIVE: We sought
       to evaluate the epidermal expression of 65 and 72 kd heat shock proteins
       (HSPs) in lesions of AIDS-associated psoriasiform dermatitis (AIDS-PD)
       and compare it with that in psoriasis vulgaris and seborrheic dermatitis
       in patients not infected with HIV. METHODS: Sections from
       paraffin-embedded blocks of biopsy specimens of AIDS-PD (eight cases),
       psoriasis vulgaris (eight cases), seborrheic dermatitis (four cases),
       and normal skin (four cases) in non-HIV-infected patients were
       immunohistochemically stained by the avidin-biotin-peroxidase method and
       two monoclonal antibodies directed against the major 65 kd HSP antigen
       (HSP65) and against 70/72 kd HSP. The intensity, distribution, and
       cellular pattern of the epidermal stain were graded and assessed
       blindly. RESULTS: The epidermal expression of HSP65 in biopsy specimens
       from AIDS-PD lesions was irregular, with less intensity and less
       tendency to perinuclear arrangement than in psoriasis or seborrheic
       dermatitis not associated with AIDS. The expression of HSP72 was also
       less intense and more uniform in AIDS-PD. CONCLUSION: The altered
       interplay of T cells and keratinocytes in a situation of immune
       derangement such as AIDS might account for the differences observed in
       the expression of HSP65 and HSP72 by keratinocytes in psoriasis and
       AIDS-PD.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS/METABOLISM/  PATHOLOGY
       Biopsy  Chaperonins/*METABOLISM  Comparative Study
       Dermatitis/ETIOLOGY/*METABOLISM/PATHOLOGY  Dermatitis,
       Seborrheic/METABOLISM/PATHOLOGY  Epidermis/*METABOLISM/PATHOLOGY
       Heat-Shock Proteins/*METABOLISM  Human  *HIV-1  Immunohistochemistry
       Psoriasis/ETIOLOGY/*METABOLISM/PATHOLOGY  Staining/METHODS  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

