       Document 0672
 DOCN  M9630672
 TI    Characterization of mouse nasal lymphocytes isolated by enzymatic
       extraction with collagenase.
 DT    9603
 AU    Asanuma H; Inaba Y; Aizawa C; Kurata T; Tamura S; Department of
       Pathology, National Institute of Health, Tokyo,; Japan.
 SO    J Immunol Methods. 1995 Nov 16;187(1):41-51. Unique Identifier :
       AIDSLINE MED/96085237
 AB    A reproducible method for isolation of mouse nasal lymphocytes was
       developed. The cells were released from tissue fragments of dissected
       mouse nose by enzyme extraction with collagenase and separated by a
       stepwise Percoll gradient centrifugation. The partially purified nasal
       lymphocyte fraction from normal BALB/c mice contained CD4+ T cells
       (18-23%), CD8+ T cells (7-10%) and B cells (20-38%), when analysed with
       a FACScan fluorescence analyser. The ratio of T to B cells and that of
       CD4+ to CD8+ T cells in the nasal cell fraction were about twice as high
       as those in Peyer's path lymphocytes. The nasal lymphocyte fraction from
       the mice infected with influenza virus was then characterized. The nasal
       lymphocytes contained a twice larger number of CD4+ and a three times
       larger number of CD8+ T cells than those of normal mice 7 days after
       infection. They produced IFN-gamma which increased after infection. They
       contained the cells secreting influenza virus-specific IgA and IgG
       antibodies 4 weeks after infection. Moreover, the nasal lymphocytes from
       infected C3H mice lysed the virus infected-target cells. These results
       suggest that this method can successfully be used for investigating
       cellular dynamics of mucosal immunity in the upper respiratory tract of
       experimental animals.
 DE    Animal  Antibodies, Viral/*ANALYSIS  Cell Separation/METHODS
       Collagenases  Cytotoxicity Tests, Immunologic  CD4-CD8 Ratio  Female
       IgA/ANALYSIS  IgG/ANALYSIS  Immunologic Techniques
       Influenza/*IMMUNOLOGY  Interferon Type II/ANALYSIS
       Interleukin-4/ANALYSIS  Lymphocyte Subsets/*IMMUNOLOGY  Mice  Mice,
       Inbred BALB C  Mice, Inbred C3H  Nasal Mucosa/*IMMUNOLOGY/VIROLOGY
       Orthomyxoviridae/*IMMUNOLOGY  Peyer's Patches/IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

