       Document 0671
 DOCN  M9630671
 TI    Immediate-type hypersensitivity response followed by a late reaction is
       induced by repeated epicutaneous application of contact sensitizing
       agents in mice.
 DT    9603
 AU    Kitagaki H; Fujisawa S; Watanabe K; Hayakawa K; Shiohara T; Department
       of Dermatology, Kyorin University School of Medicine,; Tokyo, Japan.
 SO    J Invest Dermatol. 1995 Dec;105(6):749-55. Unique Identifier : AIDSLINE
       MED/96086840
 AB    Repeated administration of antigen often leads to consequences different
       from those expected with fewer encounters with the antigen, but little
       attention has been paid to the effects of repeated epicutaneous
       application of antigens. To investigate whether repeated epicutaneous
       application of a contact-sensitizing agent that is generally thought to
       evoke a typical delayed-type hypersensitivity response could result in
       adverse or different consequences, BALB/c mice were sensitized with
       2,4,6-trinitro-1-chlorobenzine and then were repeatedly elicited on the
       original sensitized site with the same antigen for 24-48 d. Detailed
       analyses showed that the time-course of antigen-specific
       hypersensitivity responses shifted from a delayed-type hypersensitivity
       to an immediate-type response followed by a late reaction as
       epicutaneous applications were repeated, a finding different from that
       previously reported. Development of these hypersensitivity responses was
       antigen specific, and this shift was associated with epidermal
       hyperplasia, accumulation of large numbers of mast cells and CD4+ T
       cells beneath the epidermis, and elevated serum levels of
       antigen-specific IgE. The immediate-type response to
       2,4,6-trinitro-1-chlorobenzine was also induced in
       2,4,6-trinitro-1-chlorobenzine-treated, genetically mast cell-deficient
       W/Wv mice that contained significant numbers of mast cells, but not in
       similarly treated S1/S1d mice devoid of mast cells. Our experimental
       system would provide a simple, reproducible animal model for chronic
       skin inflammation induced by various antigens.
 DE    Animal  CD4-Positive T-Lymphocytes/PHYSIOLOGY  Dermatitis,
       Contact/*ETIOLOGY  Hypersensitivity, Immediate/*ETIOLOGY
       IgE/BIOSYNTHESIS  Male  Mast Cells/PHYSIOLOGY  Mice  Mice, Inbred BALB C
       Picryl Chloride/IMMUNOLOGY  Support, Non-U.S. Gov't  Th2
       Cells/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

